Anti-MEK4/MKK4 antibody [EP615Y] - BSA and Azide free (ab242052)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MEK4/MKK4 antibody [EP615Y] - BSA and Azide free
See all MEK4/MKK4 primary antibodies -
Description
Rabbit monoclonal [EP615Y] to MEK4/MKK4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MEK4/MKK4 (C terminal). The exact sequence is proprietary.
Database link: P45985 -
Positive control
- WB: Wild-type HAP1 cell lysate; HeLa and A431 cell lysates.
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General notes
ab242052 is the carrier-free version of ab33912 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab242052 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
A trial size is available to purchase for this antibody. This product was previously labelled as MEK4
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab33912 is a purified IgG. -
Clonality
Monoclonal -
Clone number
EP615Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 44 kDa. |
Target
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Function
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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Database links
- Entrez Gene: 6416 Human
- Entrez Gene: 26398 Mouse
- Entrez Gene: 287398 Rat
- Omim: 601335 Human
- SwissProt: P45985 Human
- SwissProt: P47809 Mouse
- Unigene: 514681 Human
- Unigene: 412922 Mouse
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Alternative names
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
Images
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![Western blot - Anti-MEK4/MKK4 antibody [EP615Y] - BSA and Azide free (ab242052)](https://www.abcam.com/ps/products/242/ab242052/Images/ab242052-330619-anti-mek4-antibody-ep615y-western-blot.jpg)
Western blot - Anti-MEK4/MKK4 antibody [EP615Y] - BSA and Azide free (ab242052)Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33912).
Datasheets and documents
References
ab242052 has not yet been referenced specifically in any publications.
