Overview

  • Product name

    Anti-MEK4/MKK4 (phospho S80) antibody
    See all MEK4/MKK4 primary antibodies
  • Description

    Rabbit polyclonal to MEK4/MKK4 (phospho S80)
  • Host species

    Rabbit
  • Specificity

    ab131353 detects endogenous levels of MEK4/MKK4 only when phosphorylated at Serine 80.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: NP_003001.1

  • Positive control

    • Methanol fixed HeLa cells; HepG2 cell extracts, treated with EGF; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as MEK4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131353 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • Tissue specificity

    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Information by UniProt
  • Database links

  • Alternative names

    • c Jun N terminal kinase kinase 1 antibody
    • C-JUN N-terminal kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 4 antibody
    • Dual specificity mitogen-activated protein kinase kinase 4 antibody
    • JNK Activated Kinase 1 antibody
    • JNK activating kinase 1 antibody
    • JNK-activating kinase 1 antibody
    • JNKK antibody
    • JNKK1 antibody
    • MAP kinase kinase 4 antibody
    • Map2k4 antibody
    • MAPK ERK kinase 4 antibody
    • MAPK/ERK kinase 4 antibody
    • MAPKK 4 antibody
    • MAPKK4 antibody
    • MEK 4 antibody
    • MEK4 antibody
    • Mitogen activated protein kinase kinase 4 antibody
    • MKK 4 antibody
    • MKK4 antibody
    • MP2K4_HUMAN antibody
    • PRKMK4 antibody
    • SAPK ERK kinase 1 antibody
    • SAPK/ERK kinase 1 antibody
    • SAPKK 1 antibody
    • SAPKK1 antibody
    • SEK1 antibody
    • SERK1 antibody
    • SKK1 antibody
    • Stress activated protein kinase kinase 1 antibody
    see all

Images

  • All lanes : Anti-MEK4/MKK4 (phospho S80) antibody (ab131353) at 1/500 dilution

    Lane 1 : HepG2 cell extracts, untreated
    Lane 2 : HepG2 cell extracts, treated with EGF

    Predicted band size: 44 kDa

  • Immunofluorescent analysis of methanol fixed HeLa cells labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/50 dilution (left). The image on the right is treated with blocking peptide.

References

ab131353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131353.
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