Key features and details
- Rabbit polyclonal to MEK4/MKK4 (phospho S80)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MEK4/MKK4 (phospho S80) antibody
See all MEK4/MKK4 primary antibodies
DescriptionRabbit polyclonal to MEK4/MKK4 (phospho S80)
ab131353 detects endogenous levels of MEK4/MKK4 only when phosphorylated at Serine 80.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: NP_003001.1
- Methanol fixed HeLa cells; HepG2 cell extracts, treated with EGF; Human breast carcinoma tissue.
This product was previously labelled as MEK4
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131353 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
- Information by UniProt
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
All lanes : Anti-MEK4/MKK4 (phospho S80) antibody (ab131353) at 1/500 dilution
Lane 1 : HepG2 cell extracts, untreated
Lane 2 : HepG2 cell extracts, treated with EGF
Predicted band size: 44 kDa
Immunofluorescent analysis of methanol fixed HeLa cells labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/100 dilution.
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MEK4/MKK4 (phospho S80) with ab131353 at 1/50 dilution (left). The image on the right is treated with blocking peptide.
ab131353 has been referenced in 1 publication.
- Wu X et al. Amyloid precursor protein promotes the migration and invasion of breast cancer cells by regulating the MAPK signaling pathway. Int J Mol Med 45:162-174 (2020). PubMed: 31746365