Recombinant Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y]
See all MEK4/MKK4 primary antibodies -
Description
Rabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 3T3 cell lysate treated with PDGF. IHC-P: Human muscle tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1075Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
Target
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Function
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). -
Tissue specificity
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases. - Information by UniProt
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Database links
- Entrez Gene: 6416 Human
- Entrez Gene: 26398 Mouse
- Omim: 601335 Human
- SwissProt: P45985 Human
- SwissProt: P47809 Mouse
- Unigene: 514681 Human
- Unigene: 412922 Mouse
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Alternative names
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
see all
Images
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ab52958 at 1/50 dilution staining MEK4/MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with PDGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab52958 has been referenced in 1 publication.
- Yu W et al. TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway. J Gastrointest Oncol 13:2447-2457 (2022). PubMed: 36388700