Key features and details
- Rabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MEK4/MKK4 (phospho S80) antibody [EP1075Y]
See all MEK4/MKK4 primary antibodies
DescriptionRabbit monoclonal [EP1075Y] to MEK4/MKK4 (phospho S80)
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human MEK4/MKK4 (N terminal). The exact sequence is proprietary.
- 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as MEK4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
FunctionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
- Information by UniProt
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-MEK4/MKK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with PDGF
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52958 has not yet been referenced specifically in any publications.