Overview

  • Product name

    Anti-MEK4/MKK4 (phospho T261) antibody
    See all MEK4/MKK4 primary antibodies
  • Description

    Rabbit polyclonal to MEK4/MKK4 (phospho T261)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: NP_003001.1

  • Positive control

    • Methanol fixed HeLa cells; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as MEK4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131351 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • Tissue specificity

    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Information by UniProt
  • Database links

  • Alternative names

    • c Jun N terminal kinase kinase 1 antibody
    • C-JUN N-terminal kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 4 antibody
    • Dual specificity mitogen-activated protein kinase kinase 4 antibody
    • JNK Activated Kinase 1 antibody
    • JNK activating kinase 1 antibody
    • JNK-activating kinase 1 antibody
    • JNKK antibody
    • JNKK1 antibody
    • MAP kinase kinase 4 antibody
    • Map2k4 antibody
    • MAPK ERK kinase 4 antibody
    • MAPK/ERK kinase 4 antibody
    • MAPKK 4 antibody
    • MAPKK4 antibody
    • MEK 4 antibody
    • MEK4 antibody
    • Mitogen activated protein kinase kinase 4 antibody
    • MKK 4 antibody
    • MKK4 antibody
    • MP2K4_HUMAN antibody
    • PRKMK4 antibody
    • SAPK ERK kinase 1 antibody
    • SAPK/ERK kinase 1 antibody
    • SAPKK 1 antibody
    • SAPKK1 antibody
    • SEK1 antibody
    • SERK1 antibody
    • SKK1 antibody
    • Stress activated protein kinase kinase 1 antibody
    see all

Images

  • Immunofluorescent analysis of methanol fixed HeLa cells labelling MKK4 (phospho T261) with ab131351 antibody at 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK4 (phospho T261) with ab131351 at 1/50 dilution (left). The image on the right is treated with blocking peptide.

References

This product has been referenced in:

  • Hu G  et al. MicroRNA-145 attenuates TNF-a-driven cartilage matrix degradation in osteoarthritis via direct suppression of MKK4. Cell Death Dis 8:e3140 (2017). IHC-P ; Human . Read more (PubMed: 29072705) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131351.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up