Key features and details
- Rabbit polyclonal to MEK4/MKK4 (phospho T261)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MEK4/MKK4 (phospho T261) antibody
See all MEK4/MKK4 primary antibodies
DescriptionRabbit polyclonal to MEK4/MKK4 (phospho T261)
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MEK4/MKK4 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: NP_003001.1
- Methanol fixed HeLa cells; Human breast carcinoma tissue.
This product was previously labelled as MEK4
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131351 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionDual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
- Information by UniProt
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
Immunofluorescent analysis of methanol fixed HeLa cells labelling MKK4 (phospho T261) with ab131351 antibody at 1/100 dilution.
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK4 (phospho T261) with ab131351 at 1/50 dilution (left). The image on the right is treated with blocking peptide.
ab131351 has been referenced in 1 publication.
- Hu G et al. MicroRNA-145 attenuates TNF-a-driven cartilage matrix degradation in osteoarthritis via direct suppression of MKK4. Cell Death Dis 8:e3140 (2017). IHC-P ; Human . PubMed: 29072705