Overview

  • Product name
  • Description
    Rabbit polyclonal to MEK5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-205 of Human MEK5 (Q13163)

  • Positive control
    • U87-MG whole cell lysate, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used

Target

  • Function
    Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
  • Tissue specificity
    Expressed in many adult tissues. Abundant in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Domain
    Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
  • Post-translational
    modifications
    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity mitogen activated protein kinase kinase 5 antibody
    • Dual specificity mitogen-activated protein kinase kinase 5 antibody
    • EC 2.7.12.2 antibody
    • HsT17454 antibody
    • MAP kinase kinase 5 antibody
    • MAP kinase kinase MEK5b antibody
    • MAP2K5 antibody
    • MAPK/ERK kinase 5 antibody
    • MAPKK 5 antibody
    • MAPKK5 antibody
    • MEK 5 antibody
    • mitogen-activated protein kinase kinase 5 antibody
    • MKK5 antibody
    • MP2K5_HUMAN antibody
    • PRKMK5 antibody
    • Protein kinase, mitogen-activated, kinase 5 antibody
    • SAPKK5 antibody
    • SKK5 antibody
    see all

Images

  • Anti-MEK5 antibody (ab154896) at 1/2000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling MEK5 with ab154896 at a 1/250 dilution.

References

ab154896 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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