Overview

  • Product name

  • Description

    Rabbit polyclonal to MEK5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human MEK5 aa 1-190 (N terminal).
    Database link: Q13163

  • Positive control

    • WB: Extracts from H460, BT474 cell lines. Extracts from mouse liver, rat liver and rat kidney. ICC/IF: U20S cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab210748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Function

    Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
  • Tissue specificity

    Expressed in many adult tissues. Abundant in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Domain

    Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
  • Post-translational
    modifications

    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dual specificity mitogen activated protein kinase kinase 5 antibody
    • Dual specificity mitogen-activated protein kinase kinase 5 antibody
    • EC 2.7.12.2 antibody
    • HsT17454 antibody
    • MAP kinase kinase 5 antibody
    • MAP kinase kinase MEK5b antibody
    • MAP2K5 antibody
    • MAPK/ERK kinase 5 antibody
    • MAPKK 5 antibody
    • MAPKK5 antibody
    • MEK 5 antibody
    • mitogen-activated protein kinase kinase 5 antibody
    • MKK5 antibody
    • MP2K5_HUMAN antibody
    • PRKMK5 antibody
    • Protein kinase, mitogen-activated, kinase 5 antibody
    • SAPKK5 antibody
    • SKK5 antibody
    see all

Images

  • All lanes : Anti-MEK5 antibody (ab210748) at 1/500 dilution

    Lane 1 : Extracts from H460 cell line
    Lane 2 : Extracts from BT474 cell line
    Lane 3 : Extracts from mouse liver
    Lane 4 : Extracts from rat liver
    Lane 5 : Extracts from rat kidney

    Predicted band size: 50 kDa

  • Immunofluorescence analysis of U20S cells labeling MEK5 with ab210748 (red) at a 1/50 dilution. Nuclei were counterstained with DAPI (blue).

References

ab210748 has not yet been referenced specifically in any publications.

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