Product nameAnti-MEK5 antibody
See all MEK5 primary antibodies
DescriptionRabbit polyclonal to MEK5
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human MEK5 aa 1-190 (N terminal).
Database link: Q13163
- WB: Extracts from H460, BT474 cell lines. Extracts from mouse liver, rat liver and rat kidney. ICC/IF: U20S cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab210748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/50 - 1/100.|
FunctionActs as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Tissue specificityExpressed in many adult tissues. Abundant in heart and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.
DomainBinds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
modificationsActivated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
- Dual specificity mitogen activated protein kinase kinase 5 antibody
- Dual specificity mitogen-activated protein kinase kinase 5 antibody
- EC 188.8.131.52 antibody
All lanes : Anti-MEK5 antibody (ab210748) at 1/500 dilution
Lane 1 : Extracts from H460 cell line
Lane 2 : Extracts from BT474 cell line
Lane 3 : Extracts from mouse liver
Lane 4 : Extracts from rat liver
Lane 5 : Extracts from rat kidney
Predicted band size: 50 kDa
Immunofluorescence analysis of U20S cells labeling MEK5 with ab210748 (red) at a 1/50 dilution. Nuclei were counterstained with DAPI (blue).
ab210748 has not yet been referenced specifically in any publications.