Anti-MKK6 antibody (ab154901)
Key features and details
- Rabbit polyclonal to MKK6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MKK6 antibody
See all MKK6 primary antibodies -
Description
Rabbit polyclonal to MKK6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human MKK6 aa 1-334.
Database link: P52564 -
Positive control
- WB: HEK-293T whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human N87 xenograft tissue, mouse duodenum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. -
Tissue specificity
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsWeakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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Database links
- Entrez Gene: 286883 Cow
- Entrez Gene: 5608 Human
- Entrez Gene: 26399 Mouse
- Entrez Gene: 114495 Rat
- Omim: 601254 Human
- SwissProt: Q5E9X2 Cow
- SwissProt: P52564 Human
- SwissProt: P70236 Mouse
see all -
Form
It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules. -
Alternative names
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
see all
Images
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Anti-MKK6 antibody (ab154901) at 1/1000 dilution + HEK-293T whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling MKK6 with ab154901 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human N87 xenograft tissue labeling MKK6 with ab154901 at 1/500 dilution.
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Paraffin embedded mouse duodenum tissue stained for MEK6 using ab154901 at 1/500 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154901 has been referenced in 2 publications.
- Wu G et al. CircASPH promotes KGN cells proliferation through miR-375/MAP2K6 axis in Polycystic Ovary Syndrome. J Cell Mol Med 26:1817-1825 (2022). PubMed: 33372369
- Tang B et al. PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway. Oncol Rep 41:43-56 (2019). PubMed: 30365128