Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP626Y] to MEKK2 (PE)
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-MEKK2 antibody [EP626Y] (PE)
See all MEKK2 primary antibodies
DescriptionRabbit monoclonal [EP626Y] to MEKK2 (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide. within Human MEKK2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9Y2U5
- Flow Cytometry: Jurkat cells ICC/IF: Jurkat cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab210719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde, and 80% methanol (5 min).
FunctionComponent of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Nucleus. Upon EGF stimulation, translocates into the nucleus.
- Information by UniProt
- M3K2_HUMAN antibody
- Map3k2 antibody
- MAPK/ERK kinase kinase 2 antibody
ab210719 staining MEKK2 in Jurkat cells. The cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210719 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80%methanol.
Overlay histogram showing Jurkat cells stained with ab210719 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210719, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
ab210719 has not yet been referenced specifically in any publications.