Product nameAnti-MEKK3 antibody
See all MEKK3 primary antibodies
DescriptionRabbit polyclonal to MEKK3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment, corresponding to a region within amino acids 266 and 626 of Human MEKK3 (Uniprot ID: Q99759).
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; Human Cal27 xenograft and S-G xenograft tissues.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 71 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
FunctionComponent of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.
- Information by UniProt
- M3K3_HUMAN antibody
- MAP/ERK kinase kinase 3 antibody
- MAP3K3 antibody
All lanes : Anti-MEKK3 antibody (ab154362) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S2 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human S-G xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.