Key features and details
- Rabbit polyclonal to MEKK3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MEKK3 antibody
See all MEKK3 primary antibodies
DescriptionRabbit polyclonal to MEKK3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment, corresponding to a region within amino acids 266 and 626 of Human MEKK3 (Uniprot ID: Q99759).
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; Human Cal27 xenograft and S-G xenograft tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 71 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
FunctionComponent of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.
- Information by UniProt
- M3K3_HUMAN antibody
- MAP/ERK kinase kinase 3 antibody
- MAP3K3 antibody
All lanes : Anti-MEKK3 antibody (ab154362) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S2 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human S-G xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.
ab154362 has been referenced in 1 publication.
- Tang H et al. miR-505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT-NF?B pathway in NSCLC cells. Int J Mol Med 43:1203-1216 (2019). PubMed: 30628663