Overview

  • Product name

  • Description

    Rabbit polyclonal to MEKK3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MEKK3 (internal sequence). The exact sequence is proprietary.
    Database link: Q99759

  • Positive control

    • WB: Whole cell extract of 3xDDDDK-human MEKK3-transfected HEK-293T cells. IHC-P: Human gastric cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.

Target

  • Function

    Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • M3K3_HUMAN antibody
    • MAP/ERK kinase kinase 3 antibody
    • MAP3K3 antibody
    • MAPK/ERK kinase kinase 3 antibody
    • MAPKKK3 antibody
    • MEK kinase 3 antibody
    • MEKK 3 antibody
    • MEKK3 antibody
    • Mitogen activated protein kinase kinase kinase 3 antibody
    • Mitogen-activated protein kinase kinase kinase 3 antibody
    see all

Images

  • Paraffin embedded human gastric cancer tissue stained for MEKK3 with ab227191 (1/500 dilution) in immunohistochemical analysis.

  • All lanes : Anti-MEKK3 antibody (ab227191) at 1/5000 dilution

    Lane 1 : Whole cell extract HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells
    Lane 2 : Whole cell extract of 3X DDDDK-human MEKK3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 71 kDa



    7.5 % SDS-PAGE

References

ab227191 has not yet been referenced specifically in any publications.

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