Key features and details
- Rabbit polyclonal to MEKK3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MEKK3 antibody
See all MEKK3 primary antibodies
DescriptionRabbit polyclonal to MEKK3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
Recombinant fragment within Human MEKK3 (internal sequence). The exact sequence is proprietary.
Database link: Q99759
- WB: Whole cell extract of 3xDDDDK-human MEKK3-transfected HEK-293T cells. IHC-P: Human gastric cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 71 kDa.|
FunctionComponent of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.
- Information by UniProt
- M3K3_HUMAN antibody
- MAP/ERK kinase kinase 3 antibody
- MAP3K3 antibody
Paraffin embedded human gastric cancer tissue stained for MEKK3 with ab227191 (1/500 dilution) in immunohistochemical analysis.
All lanes : Anti-MEKK3 antibody (ab227191) at 1/5000 dilution
Lane 1 : Whole cell extract HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells
Lane 2 : Whole cell extract of 3X DDDDK-human MEKK3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
7.5 % SDS-PAGE
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227191 has not yet been referenced specifically in any publications.