Product nameAnti-MEKK3 antibody
See all MEKK3 primary antibodies
DescriptionRabbit polyclonal to MEKK3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
Recombinant fragment within Human MEKK3 (internal sequence). The exact sequence is proprietary.
Database link: Q99759
- WB: Whole cell extract of 3xDDDDK-human MEKK3-transfected HEK-293T cells. IHC-P: Human gastric cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 71 kDa.|
FunctionComponent of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain.
- Information by UniProt
- M3K3_HUMAN antibody
- MAP/ERK kinase kinase 3 antibody
- MAP3K3 antibody
Paraffin embedded human gastric cancer tissue stained for MEKK3 with ab227191 (1/500 dilution) in immunohistochemical analysis.
All lanes : Anti-MEKK3 antibody (ab227191) at 1/5000 dilution
Lane 1 : Whole cell extract HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells
Lane 2 : Whole cell extract of 3X DDDDK-human MEKK3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
7.5 % SDS-PAGE
ab227191 has not yet been referenced specifically in any publications.