Overview

  • Product name
    Anti-MEKK3 antibody [ep600y]
    See all MEKK3 primary antibodies
  • Description
    Rabbit monoclonal [ep600y] to MEKK3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MEKK3 aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa cells, ovary carcinoma
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab40756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.

Target

  • Function
    Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • M3K3_HUMAN antibody
    • MAP/ERK kinase kinase 3 antibody
    • MAP3K3 antibody
    • MAPK/ERK kinase kinase 3 antibody
    • MAPKKK3 antibody
    • MEK kinase 3 antibody
    • MEKK 3 antibody
    • MEKK3 antibody
    • Mitogen activated protein kinase kinase kinase 3 antibody
    • Mitogen-activated protein kinase kinase kinase 3 antibody
    see all

Images

  • All lanes : Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution

    Lane 1 : Recombinant human MEKK2 protein (ab135009)
    Lane 2 : Recombinant human MEKK3 protein (ab85297)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 97 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunofluorescent staining of HeLa cells using ab40756 diluted 1:100
  • Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 71 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1:100

References

This product has been referenced in:

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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