Anti-MelanA antibody [EP1422Y] (ab51061)


  • Product name
    Anti-MelanA antibody [EP1422Y]
    See all MelanA primary antibodies
  • Description
    Rabbit monoclonal [EP1422Y] to MelanA
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MelanA aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16655

  • Positive control
    • Melanoma lysate or melanoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab51061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
    • Tissue specificity
      Expression is restricted to melanoma and melanocyte cell lines and retina.
    • Post-translational
    • Cellular localization
      Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen LB39 AA antibody
      • Antigen LB39-AA antibody
      • Antigen SK29 AA antibody
      • Antigen SK29-AA antibody
      • MAR1_HUMAN antibody
      • MART 1 antibody
      • MART-1 antibody
      • MART1 antibody
      • Melan A antibody
      • Melan A protein antibody
      • Melanoma antigen recognized by T cells 1 antibody
      • Melanoma antigen recognized by T-cells 1 antibody
      • MLAN A antibody
      • MLANA antibody
      • OTTHUMP00000021036 antibody
      • OTTHUMP00000021037 antibody
      • OTTHUMP00000021038 antibody
      • Protein Melan-A antibody
      see all


    • Ab51061 (1/100) staining human MelanA in melanoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunohistochemical analysis of Human malignant melanoma, staining MelanA with ab51061 at 1/20000 dilution. A Cy3-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.
    • Overlay histogram showing Malme-3M cells stained with ab51061 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51061, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-MelanA antibody [EP1422Y] (ab51061) at 1/1000 dilution + Melanoma lysate at 10 µg

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 13 kDa
      Observed band size: 20 kDa (why is the actual band size different from the predicted?)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Regazzetti C  et al. Melanocytes Sense Blue Light and Regulate Pigmentation through Opsin-3. J Invest Dermatol 138:171-178 (2018). Read more (PubMed: 28842328) »
    • Patwardhan A  et al. Routing of the RAB6 secretory pathway towards the lysosome related organelle of melanocytes. Nat Commun 8:15835 (2017). ICC/IF . Read more (PubMed: 28607494) »

    See all 15 Publications for this product

    Customer reviews and Q&As

    Merci de nous avoir contactés.

    Suite à notre conversation téléphonique, je me suis renseignée a propos de vos recherches. Apres réflexion, j’ai du mal à comprendre comment vous allez pouv...

    Read More

    Thank you for your enquiry.

    Indeed, the purification kits are designed for cleaning up small volumes of antibodies (ideallyin a volume of 100µl to 0.5ml), as you can see on the datasheet for ab102784.

    Read More

    Thank you for contacting us.

    Having checked with the lab, I am sorry to let you know that we are unable to provide these MelanA antibodies in a buffer without BSA.

    However, if you need to remove BSA and other unwanted ingredients, I...

    Read More

    You are welcome! We are always happy to help :-) Have a great day!

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Frozen sections)
    Human Tissue sections (skin, melanozytes, 10ym, OCT embedded)
    skin, melanozytes, 10ym, OCT embedded

    Dr. Christian Posch

    Verified customer

    Submitted Oct 21 2011


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