Product nameAnti-MelanA antibody [EP1422Y] - Low endotoxin, Azide free
See all MelanA primary antibodies
DescriptionRabbit monoclonal [EP1422Y] to MelanA - Low endotoxin, Azide free
Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab170090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
Please check the parent abID, ab51061, for more information on dilutions.
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionInvolved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificityExpression is restricted to melanoma and melanocyte cell lines and retina.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
- Information by UniProt
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
Anti-MelanA antibody [EP1422Y] - Low endotoxin, Azide free (ab170090) + Human melanoma lysate at 15 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 13 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blockinf buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
ab170090 has not yet been referenced specifically in any publications.