Recombinant
RabMAb

Recombinant Anti-MelanA antibody [EPR20380] (Phycoerythrin) (ab225499)

Overview

  • Product name

    Anti-MelanA antibody [EPR20380] (Phycoerythrin)
    See all MelanA primary antibodies
  • Description

    Rabbit monoclonal [EPR20380] to MelanA (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human MelanA aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16655

  • Positive control

    • Flow cyt: Malme-3M cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
  • Tissue specificity

    Expression is restricted to melanoma and melanocyte cell lines and retina.
  • Post-translational
    modifications

    Acylated.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen LB39 AA antibody
    • Antigen LB39-AA antibody
    • Antigen SK29 AA antibody
    • Antigen SK29-AA antibody
    • MAR1_HUMAN antibody
    • MART 1 antibody
    • MART-1 antibody
    • MART1 antibody
    • Melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T cells 1 antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • MLAN A antibody
    • MLANA antibody
    • OTTHUMP00000021036 antibody
    • OTTHUMP00000021037 antibody
    • OTTHUMP00000021038 antibody
    • Protein Melan-A antibody
    see all

Images

  • Overlay histogram showing Malme-3M cells stained with ab225499 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225499, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in Malme-3M cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225499 has not yet been referenced specifically in any publications.

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