Key features and details
- Mouse monoclonal [OTI3E2] to MelanA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MelanA antibody [OTI3E2]
See all MelanA primary antibodies
DescriptionMouse monoclonal [OTI3E2] to MelanA
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: MDCK and HEK293T transfected with pCMV6-ENTRY MelanA cDNA cell lysate IHC-P: Human kidney and pancreas tissues ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY MelanA
The clone number has been updated from 3E2 to OTI3E2, both clone numbers name the same clone.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab140503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/4000. Predicted molecular weight: 13 kDa.|
FunctionInvolved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Tissue specificityExpression is restricted to melanoma and melanocyte cell lines and retina.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
- Information by UniProt
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MelanA with ab140503 at 1/150 dilution.
Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY MelanA, labelling MelanA with ab140503 at 1/100 dilution.
Anti-MelanA antibody [OTI3E2] (ab140503) at 1/200 dilution + MDCK cell lysate at 10 µg
Predicted band size: 13 kDa
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling MelanA with ab140503 at 1/150 dilution.
All lanes : Anti-MelanA antibody [OTI3E2] (ab140503) at 1/4000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MelanA
Lysates/proteins at 5 µg per lane.
Predicted band size: 13 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab140503 has not yet been referenced specifically in any publications.