Anti-Melanocyte cell surface antigen antibody [Mel.2] (ab128759)

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Overview

  • Product name

    Anti-Melanocyte cell surface antigen antibody [Mel.2]

  • Description

    Mouse monoclonal [Mel.2] to Melanocyte cell surface antigen

  • Host species

    Mouse

  • Specificity

    ab128759 recognizes Melanocyte cell surface antigen expressed exclusively by Human melanocytes and pigmented Human tumours.

  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Human KW mel (2.1) melanoma cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 98% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein A

  • Clonality

    Monoclonal

  • Clone number

    Mel.2

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    It has been reported that the pathogenesis of melanoma proceeds through multiple stages, ranging from benign proliferation of melanocytic cells to acquisition of the capacity to invade tissues and metastasise. Cell surface antigens that are expressed by melanocytes are not expressed by melanoma cell lines.

  • Cellular localization

    Cell Membrane

References

ab128759 has not yet been referenced specifically in any publications.

Publishing research using ab128759? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

Thanks very much for your email and protocol. You see, I talked about your Mel.2 Ab with my boss for our Dynabeads experiment. We are really interested in this Ab, since they are targeting the outside of the melanocytes. However, there is no any reference about relevant study at this point, it would be reasonable to send us a little bit Ab as a sample for letting us to try it on. If our experiment is going well, It could be served as your future reference.

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Answer

Thank you very much for your interest in ab128759. I will be helping you while Tom is out of the office.

We do not currently have an image of ab128759 in ICC/IF and we are unable to send small aliquots because we cannot store multple vial sizes in our automated freezers.

However by participating in our AbTrial program you can now use our products without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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Question

Is it known if the epitope this antibody recognizes is extracellular? We are hoping to isolate a population of live melanocytes. We will first validate the antibody by ICC. Please send the ICC protocol.

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Answer

We have not performed any epitope mapping with this antibody. While it is known that the antibody is localized in or on the cell plasma membrane, but we do not know the specific region of binding. This antibody has been successfully tested for use in ELISA using fresh melanoma cells and in IHC-frozen at 8um thin sections. Successful fluorescence staining of melanoma cells on glass has been observed after being, washed, fixed for 30 seconds using ice cold acetone, and air dried. This is the extent of the protocol information we have available to share from localization experiments. I have included our ICC protocol with this email.

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Question

Is it known if the epitope this antibody recognizes is extracellular? We are hoping to isolate a population of live melanocytes. We will first validate the antibody by ICC. Please send the ICC protocol.

Read More
Answer

Unfortunately we have not performed any epitope mapping with this antibody. We know that the epitope is localized in or on the cell plasma membrane, but we do not know the specific region of binding.

This antibody has been successfully tested for use in ELISA using fresh melanoma cells and in IHC-frozen at 8um thin sections. Successful fluorescence staining of melanoma cells on glass has been observed by an outside laboratory after being, washed, fixed for 30 seconds using ice cold acetone, and air dried. This is the extent of the protocol information we have available to share from localization experiments.

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