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Is it known if the epitope this antibody recognizes is extracellular? We are hoping to isolate a population of live melanocytes. We will first validate the antibody by ICC. Please send the ICC protocol.
Asked on Dec 31 2012
We have not performed any epitope mapping with this antibody. While it is known that the antibody is localized in or on the cell plasma membrane, but we do not know the specific region of binding. This antibody has been successfully tested for use in ELISA using fresh melanoma cells and in IHC-frozen at 8um thin sections. Successful fluorescence staining of melanoma cells on glass has been observed after being, washed, fixed for 30 seconds using ice cold acetone, and air dried. This is the extent of the protocol information we have available to share from localization experiments. I have included our ICC protocol with this email.
Answered on Dec 31 2012