Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)](https://www.abcam.com/ps/products/0/ab732/Images/ab732-292896-anti-melanoma-antibody-hmb45-m2-7c10-m2-9e3-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)")
Key features and details
- Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3]
See all Melanoma primary antibodies -
Description
Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein
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Epitope
HMB45 and MART-1 -
Positive control
- IHC-P: Metastatic melanoma in lymph node.
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General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR313023, GR3175007]. Lot numbers higher than [GR313023] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener. -
Clonality
Monoclonal -
Clone number
HMB45 + M2-7C10 + M2-9E3 -
Myeloma
unknown -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/200.
The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
| Notes |
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IHC-P
1/100 - 1/200. The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
Target
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Relevance
Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites. -
Cellular localization
Membrane; Single-pass membrane protein -
Database links
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Omim: 605513 Human
- SwissProt: Q16655 Human
- SwissProt: Q2TA50 Mouse
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Alternative names
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)
ab732 staining Melanoma in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab732 has been referenced in 21 publications.
- Golan T et al. Adipocytes sensitize melanoma cells to environmental TGF-ß cues by repressing the expression of miR-211. Sci Signal 12:N/A (2019). PubMed: 31337739
- Li H et al. YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep 29:3200-3211.e4 (2019). PubMed: 31801083
- Han N et al. Enhanced IL-34 expression in Nivolumab-resistant metastatic melanoma. Inflamm Regen 38:3 (2018). PubMed: 29515691
- Malcov-Brog H et al. UV-Protection Timer Controls Linkage between Stress and Pigmentation Skin Protection Systems. Mol Cell 72:444-456.e7 (2018). PubMed: 30401431
- Yanaki M et al. In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation. Integr Cancer Ther 17:646-653 (2018). PubMed: 29665734