Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)
- Datasheet
- Specific References (17)
- Protocols
Overview
-
Product nameAnti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3]
See all Melanoma primary antibodies -
DescriptionMouse monoclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma
-
Host speciesMouse
-
Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Frmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein
-
EpitopeHMB45 and MART-1
-
Positive control
- IHC-P: Metastatic melanoma in lymph node.
-
General notes
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR313023, GR3175007]. Lot numbers higher than [GR313023] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
PurityTissue culture supernatant
-
Primary antibody notesHMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
-
ClonalityMonoclonal
-
Clone numberHMB45 + M2-7C10 + M2-9E3
-
Myelomaunknown
-
IsotypeIgG2b
-
Light chain typekappa
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. PubMed: 22147606 ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P | 1/100 - 1/200. The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
|
| IHC-Fr | Use at an assay dependent concentration. |
Target
-
RelevanceMalignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
-
Cellular localizationMembrane; Single-pass membrane protein
-
Database links
- Entrez Gene: 22173 Mouse
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Entrez Gene: 308800 Rat
- Omim: 605513 Human
- Omim: 606933 Human
- SwissProt: P14679 Human
- SwissProt: Q16655 Human
see all -
Alternative names
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)](https://www.abcam.com/ps/products/0/ab732/Images/ab732-292896-ab732.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)ab732 staining Melanoma in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)](https://www.abcam.com/ps/products/0/ab732/Images/ab732_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)This image is courtesy of an anonymous Abreviewab732 staining murine melanoma metastasis tissue sections by IHC-P. Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated for 18 hours at 4°C. An Alexa Fluor 488® conjugated goat anti-mouse was used as the secondary
Confocal image shows melanoma in magenta, vessels in red (Collagen Type IV, ab19808), and melanin in cyan. Tissue is perfusion-fixed 15µm cryostat sections mounted on slides (i.e., lightly fixed, but not paraffin embedded).
References
This product has been referenced in:
- Han N et al. Enhanced IL-34 expression in Nivolumab-resistant metastatic melanoma. Inflamm Regen 38:3 (2018). Read more (PubMed: 29515691) »
- Kong X et al. Cancer drug addiction is relayed by an ERK2-dependent phenotype switch. Nature 550:270-274 (2017). Read more (PubMed: 28976960) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"