• Product name

    Anti-Melanoma gp100 antibody
    See all Melanoma gp100 primary antibodies
  • Description

    Goat polyclonal to Melanoma gp100
  • Host species

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 647-660 of Human Melanoma gp100

  • Positive control

    • Human skin lysate



Our Abpromise guarantee covers the use of ab52058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
    • Tissue specificity

      Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
    • Sequence similarities

      Belongs to the PMEL/NMB family.
      Contains 1 PKD domain.
    • Domain

      The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
      The lumenal domain is necessary for correct processing and trafficking to melanosomes.
    • Post-translational

      A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
      N-glycosylated. O-glycosylated; contains sialic acid.
    • Cellular localization

      Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • 95 kDa melanocyte specific secreted glycoprotein antibody
      • 95 kDa melanocyte-specific secreted glycoprotein antibody
      • D12S53E antibody
      • gp100 antibody
      • M-beta antibody
      • ME20 antibody
      • ME20 M/ME20 S antibody
      • ME20-M antibody
      • ME20-S antibody
      • ME20M antibody
      • ME20M/ME20S antibody
      • ME20S antibody
      • Melanocyte lineage specific antigen GP100 antibody
      • Melanocyte protein mel 17 antibody
      • Melanocyte protein Pmel 17 antibody
      • Melanocyte protein Pmel 17 precursor antibody
      • Melanocytes lineage-specific antigen GP100 antibody
      • Melanoma associated ME20 antigen antibody
      • Melanoma gp100 antibody
      • Melanoma-associated ME20 antigen antibody
      • Melanosomal matrix protein 17 antibody
      • Melanosomal matrix protein17 antibody
      • P1 antibody
      • p100 antibody
      • p26 antibody
      • PMEL 17 antibody
      • PMEL antibody
      • PMEL_HUMAN antibody
      • PMEL17 antibody
      • Premelanosome protein antibody
      • Secreted melanoma-associated ME20 antigen antibody
      • SI antibody
      • SIL antibody
      • SILV antibody
      • Silver (mouse homolog) like antibody
      • Silver homolog antibody
      • Silver locus protein homolog antibody
      • Silver, mouse, homolog of antibody
      see all


    • Anti-Melanoma gp100 antibody (ab52058) at 1 µg/ml + Human Skin lysate at 35 µg

      Predicted band size: 70 kDa
      Additional bands at: 26 kDa (possible immature (unprocessed))

      Primary incubation was 1 hour. Detected by chemiluminescence. The 26kDa band observed in Human Skin lysate corresponds to the precusor protein M-beta fragment (Leonhardt et al, Mol Biol Cell. 2013 Apr;24(7):964-81.PMID: 23389629).

    • All lanes : Anti-Melanoma gp100 antibody (ab52058) at 1 µg/ml

      Lane 1 : Cell lysates prepared from mock transfected HEK293 cells
      Lane 2 : Cell lysates prepared from melanoma gp100 transfected HEK293 cells

      Predicted band size: 70 kDa


    This product has been referenced in:

    • Hee JS  et al. Melanosomal formation of PMEL core amyloid is driven by aromatic residues. Sci Rep 7:44064 (2017). Read more (PubMed: 28272432) »
    • Shimshek DR  et al. Pharmacological BACE1 and BACE2 inhibition induces hair depigmentation by inhibiting PMEL17 processing in mice. Sci Rep 6:21917 (2016). WB . Read more (PubMed: 26912421) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Frozen sections)
    Mouse Tissue sections (Subcutaneous melanoma primary tumor B16F10)
    Yes - 0,5% Triton X-100, 20min
    Subcutaneous melanoma primary tumor B16F10
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Feb 08 2018


    Thank you for your inquiry.

    I can confirm that it is possible to assay proteins via ELISA.

    We have the following products that will be suitable for gp100:

    ab52058 (Anti-Melanoma gp100 antibody) is tested and guaranteed for ELISA and human gp100.

    https://www.abcam.com/index.html?datasheet=52058 (or use the following: https://www.abcam.com/index.html?datasheet=52058).

    In order to perform an ELISA the liposomes loaded with gp100 will have to be lysed and then the plates coated with the protein. I can also strongly suiggest to use a standard/positive control with a known concentration for this.

    The following ab132146 (Melanoma gp100 protein (Tagged)) protein is suitable as standard:

    https://www.abcam.com/index.html?datasheet=132146 (or use the following: https://www.abcam.com/index.html?datasheet=132146).

    Protocols can be found here:



    Unfortunately, we do not have antibodies against CpG oligonucleotides. Since gp100 and CpG oligonucleotides are used for immunisations, I am not sure that the antiboduy and protein above it the correct one for you. Please cross check if it is the same gp100!

    I hope this information is helpful and wish you good luck for your research.

    Read More


    I am very pleased to hear you would like to accept our offer and test ab52058 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More


    Thank you for your enquiry and your interest in our products.

    I regret to inform you that the immunogen used to raise this antibody is not commercially available so it is not in our catalogue.


    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More
    Western blot
    Mouse Cell lysate - whole cell (HEK293 transfected with mouse GP100 plasmid)
    Loading amount
    10000 cells
    HEK293 transfected with mouse GP100 plasmid
    44 hrs post transfection
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Sep 29 2010

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