Anti-Melanoma Inhibitory Activity antibody (ab110612)
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Overview
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Product name
Anti-Melanoma Inhibitory Activity antibody
See all Melanoma Inhibitory Activity primary antibodies -
Description
Rabbit polyclonal to Melanoma Inhibitory Activity -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, Indirect ELISA, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Highly pure (>98%) recombinant Human Melanoma Inhibitory Activity
Properties
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Form
Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab110612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| Indirect ELISA | Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Melanoma Inhibitory Activity. | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin) (ab84235). Using ab84235 as the detection antibody and ab110612 as the capture antibody, the detection limit for recombinant Human Melanoma Inhibitory Activity is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Human Melanoma Inhibitory Activity is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. -
Tissue specificity
All malignant melanoma cell lines tested and infrequently in glioma cell lines. -
Sequence similarities
Belongs to the MIA/OTOR family.
Contains 1 SH3 domain. -
Post-translational
modificationsMay possess two intramolecular disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8190 Human
- Omim: 601340 Human
- SwissProt: Q16674 Human
- Unigene: 646364 Human
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Alternative names
- Cartilage derived retinoic acid sensitive protein antibody
- CD RAP antibody
- CDRAP antibody
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Images
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Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (ab110612)This image is courtesy of an anonymous Abreviewab110612 staining Melanoma Inhibitory Activity in Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/1000 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Datasheets and documents
References
ab110612 has not yet been referenced specifically in any publications.
