Key features and details
- Goat polyclonal to Melanoma Inhibitory Activity
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Melanoma Inhibitory Activity antibody
See all Melanoma Inhibitory Activity primary antibodies
DescriptionGoat polyclonal to Melanoma Inhibitory Activity
SpecificityThe immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Horse, Guinea pig, Cynomolgus monkey, Rhesus monkey, Gorilla
- Human Glioma lysates
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab166932 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab166932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 14 kDa.
1 hour primary incubation is recommended for this product.
FunctionElicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Tissue specificityAll malignant melanoma cell lines tested and infrequently in glioma cell lines.
Sequence similaritiesBelongs to the MIA/OTOR family.
Contains 1 SH3 domain.
modificationsMay possess two intramolecular disulfide bonds.
- Information by UniProt
- Cartilage derived retinoic acid sensitive protein antibody
- CD RAP antibody
- CDRAP antibody
Anti-Melanoma Inhibitory Activity antibody (ab166932) at 1 µg + Human Glioma lysates (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 14 kDa
Primary incubation was 1 hour.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) staining Melanoma Inhibitory Activity in Human spleen with ab166932 at 5 µg/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) staining Melanoma Inhibitory Activity in Human Skeletal Muscle with ab166932 at 5 µg/ml
ab166932 has not yet been referenced specifically in any publications.