Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Melanoma Inhibitory Activity antibody (Biotin)
See all Melanoma Inhibitory Activity primary antibodies -
Description
Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin) -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant full length Human Melanoma Inhibitory Activity
Properties
-
Form
Lyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
Purification notes
ab84235 was purified by affinity chromatography and then biotinylated. Filter sterilised. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab84235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | ||
| WB | ||
| Flow Cyt |
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detection limit for recombinant human protein is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 15 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. -
Tissue specificity
All malignant melanoma cell lines tested and infrequently in glioma cell lines. -
Sequence similarities
Belongs to the MIA/OTOR family.
Contains 1 SH3 domain. -
Post-translational
modificationsMay possess two intramolecular disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 8190 Human
- Omim: 601340 Human
- SwissProt: Q16674 Human
- Unigene: 646364 Human
-
Alternative names
- Cartilage derived retinoic acid sensitive protein antibody
- CD RAP antibody
- CDRAP antibody
see all
Images
-
Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)This image is courtesy of an anonymous Abreviewab84235 staining Melanoma Inhibitory Activity in the Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Datasheets and documents
References
ab84235 has not yet been referenced specifically in any publications.
