Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)

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Key features and details

  • Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin)
  • Suitable for: Sandwich ELISA, WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Anti-Melanoma Inhibitory Activity antibody (Biotin)
    See all Melanoma Inhibitory Activity primary antibodies

  • Description

    Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin)

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Highly pure (>98%) recombinant full length Human Melanoma Inhibitory Activity

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml.

Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa.

Detection limit for recombinant human protein is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function

    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

  • Tissue specificity

    All malignant melanoma cell lines tested and infrequently in glioma cell lines.

  • Sequence similarities

    Belongs to the MIA/OTOR family.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    May possess two intramolecular disulfide bonds.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cartilage derived retinoic acid sensitive protein antibody
      • CD RAP antibody
      • CDRAP antibody
      • Melanoma derived growth regulatory protein precursor antibody
      • Melanoma inhibitory activity protein antibody
      • Melanoma-derived growth regulatory protein antibody
      • Mia antibody
      • MIA_HUMAN antibody
      see all

    References (0)

    Publishing research using ab84235? Please let us know so that we can cite the reference in this datasheet.

    ab84235 has not yet been referenced specifically in any publications.

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    Flow Cytometry abreview for Anti-Melanoma Inhibitory Activity antibody (Biotin)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - Saponine
    Sample
    Human Cell (melanoma cell line)
    Specification
    melanoma cell line
    Gating Strategy
    cell line only
    Preparation
    Cell harvesting/tissue preparation method: EDTA (Versen) for harvesting cell
    Sample buffer: PBS
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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