Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Melanoma Inhibitory Activity antibody (Biotin)
See all Melanoma Inhibitory Activity primary antibodies -
DescriptionRabbit polyclonal to Melanoma Inhibitory Activity (Biotin)
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Host speciesRabbit
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ConjugationBiotin
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Tested applicationsSuitable for: Sandwich ELISA, WB, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant full length Human Melanoma Inhibitory Activity
Properties
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FormLyophilised:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
Purification notesab84235 was purified by affinity chromatography and then biotinylated. Filter sterilised.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab84235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | ||
| WB | ||
| Flow Cyt |
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detection limit for recombinant human protein is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 15 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionElicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
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Tissue specificityAll malignant melanoma cell lines tested and infrequently in glioma cell lines.
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Sequence similaritiesBelongs to the MIA/OTOR family.
Contains 1 SH3 domain. -
Post-translational
modificationsMay possess two intramolecular disulfide bonds. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 8190 Human
- Omim: 601340 Human
- SwissProt: Q16674 Human
- Unigene: 646364 Human
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Alternative names
- Cartilage derived retinoic acid sensitive protein antibody
- CD RAP antibody
- CDRAP antibody
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Images
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Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)This image is courtesy of an anonymous Abreviewab84235 staining Melanoma Inhibitory Activity in the Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
References
ab84235 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"