Product nameAnti-Melanoma Inhibitory Activity antibody [EPR15597]
See all Melanoma Inhibitory Activity primary antibodies
DescriptionRabbit monoclonal [EPR15597] to Melanoma Inhibitory Activity
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Melanoma Inhibitory Activity aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q16674
- WM-266-4 and A375 cell lysates; Human melanoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionElicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Tissue specificityAll malignant melanoma cell lines tested and infrequently in glioma cell lines.
Sequence similaritiesBelongs to the MIA/OTOR family.
Contains 1 SH3 domain.
modificationsMay possess two intramolecular disulfide bonds.
- Information by UniProt
- Cartilage derived retinoic acid sensitive protein antibody
- CD RAP antibody
- CDRAP antibody
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling Melanoma Inhibitory Activity with ab186731 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
All lanes : Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731) at 1/1000 dilution
Lane 1 : WM-266-4 cell lysate
Lane 2 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
ab186731 has not yet been referenced specifically in any publications.