Recombinant
RabMAb

Recombinant Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731)

Overview

  • Product name

    Anti-Melanoma Inhibitory Activity antibody [EPR15597]
    See all Melanoma Inhibitory Activity primary antibodies
  • Description

    Rabbit monoclonal [EPR15597] to Melanoma Inhibitory Activity
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Melanoma Inhibitory Activity aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16674

  • Positive control

    • WM-266-4 and A375 cell lysates; Human melanoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 12 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
  • Tissue specificity

    All malignant melanoma cell lines tested and infrequently in glioma cell lines.
  • Sequence similarities

    Belongs to the MIA/OTOR family.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    May possess two intramolecular disulfide bonds.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cartilage derived retinoic acid sensitive protein antibody
    • CD RAP antibody
    • CDRAP antibody
    • Melanoma derived growth regulatory protein precursor antibody
    • Melanoma inhibitory activity protein antibody
    • Melanoma-derived growth regulatory protein antibody
    • Mia antibody
    • MIA_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling Melanoma Inhibitory Activity with ab186731 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • All lanes : Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731) at 1/1000 dilution

    Lane 1 : WM-266-4 cell lysate
    Lane 2 : A375 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

References

ab186731 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186731.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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