Overview

  • Product name

    Anti-Melanophilin antibody
    See all Melanophilin primary antibodies
  • Description

    Goat polyclonal to Melanophilin
  • Host species

    Goat
  • Specificity

    ab2716 recognises Melanophilin.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Melanophilin aa 577-590 (C terminal).
    Sequence:

    ARHIFAKPVMAQQP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 0.5 µg/ml.

Target

  • Function

    Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
  • Involvement in disease

    Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations.
  • Sequence similarities

    Contains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2210418F23Rik antibody
    • 5031433I09Rik antibody
    • AW228792 antibody
    • D1Wsu84e antibody
    • Exophilin 3 antibody
    • Exophilin-3 antibody
    • l(1)-3Rk antibody
    • l1Rk3 antibody
    • Leaden antibody
    • Leaden protein antibody
    • ln antibody
    • Melanophilin antibody
    • MELPH_HUMAN antibody
    • MGC2771 antibody
    • MGC59733 antibody
    • Mlph antibody
    • Slac 2a antibody
    • SlaC2-a antibody
    • Slp homolog lacking C2 domains a antibody
    • Synaptotagmin like protein 2a antibody
    • Synaptotagmin like protein lacking C2 domains A antibody
    • Synaptotagmin-like protein 2a antibody
    see all

Images

  • All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml

    Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
    Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice

    Predicted band size: 65 kDa
    Observed band size: 81 kDa
    why is the actual band size different from the predicted?



    ab2716 staining (3µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence.
  • Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.

References

This product has been referenced in:

  • Kudo M  et al. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS One 12:e0171513 (2017). Read more (PubMed: 28182699) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

This antibody has not yet been tested for use in IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

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