Melanophilin overexpression 293T lysate (whole cell) (ab94249)
Overview
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Product name
Melanophilin overexpression 293T lysate (whole cell) -
General notes
ab94249 is a 293T cell transfected lysate in which Human Melanophilin has been transiently over-expressed using a pCMV-Melanophilin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. Disease: Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. Similarity: Contains 1 FYVE-type zinc finger. Contains 1 RabBD (Rab-binding) domain.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent dilution.
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| Notes |
|---|
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WB
Use at an assay dependent dilution. |
Images
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SDS-PAGE - Melanophilin overexpression 293T lysate (whole cell) (ab94249)ab94249 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Melanophilin overexpression 293T lysate (whole cell) (ab94249)All lanes : Anti-Melanophilin antibody (ab89169) at 1/500 dilution
Lane 1 :Melanophilin overexpression 293T lysate (whole cell) (ab94249)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94249 has not yet been referenced specifically in any publications.