Overview

  • Product name
  • Description
    Rabbit polyclonal to Melanopsin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KMNSPSESRVPSSLTQDPSF

    , corresponding to amino acids 1-19 of Rat Melanopsin.

  • Positive control
    • ICC: rat retinal ganglion cells WB: overexpressed melanopsin in HEK293 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.
  • Tissue specificity
    Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Cellular localization
    Cell membrane. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Melanopsin antibody
    • MGC142118 antibody
    • MOP antibody
    • OPN 4 antibody
    • OPN4 antibody
    • OPN4_HUMAN antibody
    • Opsin 4 antibody
    • Opsin-4 antibody
    • Opsin4 antibody
    see all

Images

  • Immunofluorescence and immunocytochemistry staining of Melanopsin in rat retinal ganglion cells (RGCs) can be performed using ab19306.

  • By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated rat melanopsin from HEK 293 cells.

References

This product has been referenced in:
  • Benedetto MM  et al. Non-Visual Photopigments Effects of Constant Light-Emitting Diode Light Exposure on the Inner Retina of Wistar Rats. Front Neurol 8:417 (2017). Read more (PubMed: 28871236) »
  • Boudard DL  et al. Loss of photic entrainment at low illuminances in rats with acute photoreceptor degeneration. Eur J Neurosci 30:1527-36 (2009). Read more (PubMed: 19821841) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply. I am glad you found the information I sent helpful. I hope you obtain the staining you are hoping for with the Melanopsin antibody you have purchased. If I can be of any further assistance, please do not hesitate to let me know.

Until then, I wish you all the best with your research.

Read More

Answer

Thank you for contacting us yesterday in regards to the Melanopsin antibodies available from our catalogue. I am sorry for the delay in my reply.

As discussed over the phone, I have checked if we have any images available for the results obtained with ab65641 in IHC. Unfortunately the lab have informed me that we do not have any to share at present. I can understand that this may be of concern to you with the rare tissue you are using. I would suggest that if the tissue is very important, that you used some adult tissue first which is not as precious to optimise the protocol, then use it with your fetal tissue if you are happy with the results. If you would be interested, I am able to offer you a testing discount if you would be interested in trying this antibody.

This offer means that if you purchase ab65641 as normal, test the antibody with your human samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab65641 (£227) off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab65641.

I also checked the antibodies ab19306 and ab19383 which have both been used in IHC with rat tissues and have images showing on the datasheet. However, they are not likely to cross react with your human tissue. The homology of the immunogen used to raise ab137319 is only 66% whilst that of ab19306 is even lower. I would therefore not recommend trying these antibodies.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your enquiry. I am sorry to hear you are having difficulty obtaining satisfactory results from this antibody (ab19306). This antibody has been tested on total protein extracts of HEK293 cells with or without a c-myc tag (to verify the specificity of the antibody). From overexpressed melanopsin, a band is observed at the expected molecular mass (~53 kDa). There us also a larger band (~85 kDa) which is glycosylated melanopsin (this disappears under deglycosylated conditions). The antibody is known to give bands of smaller molecular weight from intrinsically degraded expressed melanopsin protein. I can suggest that the band you are observing at 43kDa is the non-glycosylated melanopsin. Although this is 10kDa less than the expected size, molecular weight markers are not accurate enough to give a precise molecular weight. The larger glycosylated band at 85kDa has probably disappeared under reducing conditions due to affects of the reducing and denaturing process on the glycosylation. Ensure you are reducing and denaturing adequately so the protein runs at the correct molecular weight. I hope this information is helpful. However, if you are still concerned about your results, I have enclosed a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some assistance. https://www.abcam.com/index.html?section=western&pageconfig=technical&mode=questionaire We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. Please do not hesitate to contact us again should you require further assistance. Good luck with your experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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