Key features and details
- Rabbit polyclonal to Melanopsin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionPhotoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.
Tissue specificityEye. Expression is restricted within the ganglion and amacrine cell layers of the retina.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Cellular localizationCell membrane. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.
- Information by UniProt
- Melanopsin antibody
- MGC142118 antibody
- MOP antibody
ab19306 has been referenced in 4 publications.
- Benedetto MM et al. Non-Visual Photopigments Effects of Constant Light-Emitting Diode Light Exposure on the Inner Retina of Wistar Rats. Front Neurol 8:417 (2017). PubMed: 28871236
- Liu M et al. Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Mol Med Rep 13:321-6 (2016). PubMed: 26572076
- Jain V et al. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS One 11:e0157226 (2016). PubMed: 27295136
- Boudard DL et al. Loss of photic entrainment at low illuminances in rats with acute photoreceptor degeneration. Eur J Neurosci 30:1527-36 (2009). PubMed: 19821841