Overview

  • Product name
  • Description
    Rabbit polyclonal to Melanopsin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Melanopsin aa 455-474.
    Sequence:

    EQKSKTPKTKRHLPSLDRRM

  • Positive control
    • Rat retinal ganglion cells (RGCs) for immunofluorescence and immunohistochemistry.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Not recommended for the detection of endogenously expressed protein.

Target

Images

  • Immunofluorescence staining of melanopsin in rat retinal ganglion cells using ab19383. 

References

This product has been referenced in:
  • Diessler S  et al. Rai1 frees mice from the repression of active wake behaviors by light. Elife 6:N/A (2017). Read more (PubMed: 28548639) »
  • Man PS  et al. Rhythmic expression of an egr-1 transgene in rats distinguishes two populations of photoreceptor cells in the retinal outer nuclear layer. Mol Vis 14:1176-86 (2008). IHC . Read more (PubMed: 18587494) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. Further to your technical enquiry I have been in touch with my colleague who removed mouse reactivity for this antibody. Apparently the customer who complained about the murine reactivity used the antibody up to concentration of 5 ug/mL (50 times the recommended dilution) and did not see any band at 53 or 85 kD whether he incubated overnight or not, and whether he incubated in 5% milk or no milk. Upon further inspection the immunogen for ab19383 does not appear to be particularly similar to the mouse sequence. Following a ClustalW alignment there appears to be only a string of 5 amino acids that were identical. Given the minimal immunogen similarity and the reliability of the source of the complaint we feel that at this time we cannot support this antibody as having cross reactivity against mouse. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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