Recombinant
RabMAb

Recombinant Anti-Melatonin antibody [EPPTX-R10-35-7] - BSA and Azide free (ab243567)

Overview

  • Product name

    Anti-Melatonin antibody [EPPTX-R10-35-7] - BSA and Azide free
    See all Melatonin primary antibodies
  • Description

    Rabbit monoclonal [EPPTX-R10-35-7] to Melatonin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Chemical/ Small Molecule corresponding to Melatonin. (Melatonin-BSA).

  • General notes

    Ab243567 is the carrier-free version of ab241066. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab243567 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Melatonin, the principal hormone of the pineal gland, is also produced by the retina, lens and GI tract. It is naturally synthesized from the amino acid tryptophan (via synthesis of serotonin) by the enzyme 5-hydroxyindole-O-methyltransferase. Production of melatonin by the pineal gland is under the influence of the suprachiasmatic nucleus of the hypothalamus (SCN -the site of a circadian clock) which receives information from the retina about the daily pattern of light and darkness. Melatonin can alter the timing of mammalian circadian rhythms, as well as regulate the reproductive alterations that occur in response to changes in day length in seasonally breeding mammals. Melatonin is also an extremely powerful antioxidant, with a particular role in the protection of nuclear and mitochondrial DNA. Therapeutically there may be many potential uses for melatonin such as in the treatment of various forms of some forms of depression, cancer, HIV, plus other viral diseases. Currently, Melatonin is a popular therapy for jet-lag and disturbances of sleep.
  • Cellular localization

    Secreted
  • Alternative names

    • 5 methoxy N acetyltryptamine antibody
    • Melatonin antibody
    • N acetyl 5 methoxytryptamine antibody

Images

  • Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions of Melatonin (0, 3, 10, 30, 100, 300pg/mL) was added (25μL). This was followed by adding 50μL HRP-conjugated Melatonin and 4.0-4.5ng/mL of ab241066 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 5 minutes.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241066).
  • ELISA - Anti-Melatonin antibody [EPPTX-R10-35-7] (ab241066) at 4.0 ng/ml.

    ab241066 is specific to Melatonin and the percent cross reactivity with other compounds is demonstrated in the table.

    This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241066).

References

ab243567 has not yet been referenced specifically in any publications.

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