Anti-Melatonin Receptor 1B/MTNR1B antibody - N-terminal (ab229544)
Key features and details
- Rabbit polyclonal to Melatonin Receptor 1B/MTNR1B - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Melatonin Receptor 1B/MTNR1B antibody - N-terminal
See all Melatonin Receptor 1B/MTNR1B primary antibodies -
Description
Rabbit polyclonal to Melatonin Receptor 1B/MTNR1B - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Melatonin Receptor 1B/MTNR1B (N terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P49286 -
Positive control
- WB: K562, THP-1, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysates.
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General notes
This product was previously labelled as Melatonin Receptor 1B
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
Target
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Function
High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. -
Tissue specificity
Expressed in retina and less in brain and hippocampus. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4544 Human
- Omim: 600804 Human
- SwissProt: P49286 Human
- Unigene: 569039 Human
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Alternative names
- FGQTL2 antibody
- Mel 1b antibody
- Mel 1b melatonin receptor antibody
see all
Images
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Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody - N-terminal (ab229544)All lanes : Anti-Melatonin Receptor 1B/MTNR1B antibody - N-terminal (ab229544) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa12% SDS-PAGE gel.
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Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody - N-terminal (ab229544)All lanes : Anti-Melatonin Receptor 1B/MTNR1B antibody - N-terminal (ab229544) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 whole cell lysate
Lane 4 : SK-N-AS whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa12% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab229544 has been referenced in 1 publication.
- Jablonska K et al. Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC). Cancers (Basel) 11:N/A (2019). PubMed: 31319607
