Anti-MELK antibody [2G2] (ab129373)
Key features and details
- Mouse monoclonal [2G2] to MELK
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MELK antibody [2G2]
See all MELK primary antibodies -
Description
Mouse monoclonal [2G2] to MELK -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis -
Immunogen
Synthetic peptide:
C-VYKRLVEDILSSCKV
(with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680). -
Positive control
- HepG2 cells and MCF-7 cells. Human liver cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR248807-19 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2G2 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/1000.
|
|
Flow Cyt |
1/200 - 1/400.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/200 - 1/1000.
|
Notes |
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IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. -
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9833 Human
- Entrez Gene: 17279 Mouse
- Omim: 607025 Human
- SwissProt: Q14680 Human
- SwissProt: Q61846 Mouse
- Unigene: 184339 Human
- Unigene: 268668 Mouse
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Alternative names
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
see all
Images
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ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
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ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).
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ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
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ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab129373 has been referenced in 5 publications.
- Chen Z et al. Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma. Dig Surg 39:24-31 (2022). PubMed: 34788753
- Zhu Y et al. Identification of potential circular RNA biomarkers in lung adenocarcinoma: A bioinformatics analysis and retrospective clinical study. Oncol Lett 23:144 (2022). PubMed: 35340554
- Yang H et al. MELK is a prognostic biomarker and correlated with immune infiltration in glioma. Front Neurol 13:977180 (2022). PubMed: 36353126
- Meel MH et al. MEK/MELK inhibition and blood-brain barrier deficiencies in atypical teratoid/rhabdoid tumors. Neuro Oncol 22:58-69 (2020). PubMed: 31504799
- Chen S et al. Maternal embryonic leucine zipper kinase: A novel biomarker and a potential therapeutic target in lung adenocarcinoma. Oncol Lett 20:147 (2020). PubMed: 32934715