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    melk-antibody-ab245574.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-MELK antibody (ab245574)

  • Datasheet
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Abpromise

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Western blot - Anti-MELK antibody (ab245574)
  • Immunoprecipitation - Anti-MELK antibody (ab245574)

Key features and details

  • Rabbit polyclonal to MELK
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human MELK protein (Active) (ab204155)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MELK antibody
    See all MELK primary antibodies
  • Description

    Rabbit polyclonal to MELK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MELK aa 425-475. The exact sequence is proprietary. NP_055606.1
    Database link: Q14680

  • Positive control

    • WB: HeLa and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245574 was affinity purified using an epitope specific to MELK immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human MELK protein (Active) (ab204155)
  • Related Products

    • Recombinant human MELK protein (Active) (ab204155)

Applications

Our Abpromise guarantee covers the use of ab245574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
  • Tissue specificity

    Expressed in placenta, kidney, thymus, testis, ovary and intestine.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q14680 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9833 Human
    • Omim: 607025 Human
    • SwissProt: Q14680 Human
    • Unigene: 184339 Human
    • Alternative names

      • AI327312 antibody
      • hMELK antibody
      • HPK 38 antibody
      • hPK38 antibody
      • KIAA0175 antibody
      • Likely ortholog of maternal embryonic leucine zipper kinase antibody
      • Maternal embryonic leucine zipper kinase antibody
      • MELK antibody
      • MELK_HUMAN antibody
      • mKIAA0175 antibody
      • MPK38 antibody
      • OTTHUMP00000021377 antibody
      • OTTHUMP00000046113 antibody
      • pEg3 kinase antibody
      • Protein kinase Eg3 antibody
      • Protein kinase PK38 antibody
      • RP23 382O11.1 antibody
      • Tyrosine protein kinase MELK antibody
      see all

    Images

    • Western blot - Anti-MELK antibody (ab245574)
      Western blot - Anti-MELK antibody (ab245574)
      All lanes : Anti-MELK antibody (ab245574) at 0.1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 75 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-MELK antibody (ab245574)
      Immunoprecipitation - Anti-MELK antibody (ab245574)

      MELK was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

      ab245574 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.

      Lane 1: ab245574 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245574? Please let us know so that we can cite the reference in this datasheet.

    ab245574 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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