Key features and details
- Rabbit polyclonal to MELK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MELK antibody
See all MELK primary antibodies
DescriptionRabbit polyclonal to MELK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MELK aa 425-475. The exact sequence is proprietary. NP_055606.1
Database link: Q14680
- WB: HeLa and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245574 was affinity purified using an epitope specific to MELK immobilized on solid support.
Our Abpromise guarantee covers the use of ab245574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPhosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Tissue specificityExpressed in placenta, kidney, thymus, testis, ovary and intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
- Information by UniProt
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
All lanes : Anti-MELK antibody (ab245574) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30 seconds
MELK was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245574 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245574 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245574 has not yet been referenced specifically in any publications.