Product nameAnti-MELK antibody
See all MELK primary antibodies
DescriptionRabbit polyclonal to MELK
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MELK conjugated to keyhole limpet haemocyanin.
Database link: Q14680
- WB: MCF7 and NIH3T3 whole cell lysate. Mouse and rat liver tissue lysate. IHC-P: Human lung cancer tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab273015 in the following tested applications.
|WB||1/500 - 1/1000. Predicted molecular weight: 74 kDa.|
|IHC-P||1/100 - 1/200.|
|ICC/IF||1/100 - 1/500.|
FunctionPhosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Tissue specificityExpressed in placenta, kidney, thymus, testis, ovary and intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
- Information by UniProt
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
All lanes : Anti-MELK antibody (ab273015) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat liver tissue lysate
Predicted band size: 74 kDa
Human lung cancer tissue stained for MELK using ab273015 at 1/100 dilution in immunohistochemical analysis.
Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling MELK (red) using ab273015 at 1/100 dilution.
ab273015 has not yet been referenced specifically in any publications.