Anti-Melphalan antibody [Amp4/42] (ab97554)
Key features and details
- Mouse monoclonal [Amp4/42] to Melphalan
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-Melphalan antibody [Amp4/42] -
Description
Mouse monoclonal [Amp4/42] to Melphalan -
Host species
Mouse -
Specificity
This antibody recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali. The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and this antibody appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Melphalan. DNA/ melphalan alkylated DNA.
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Positive control
- DNA/ melphalan alkylated DNA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Amp4/42 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent dilution.
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Notes |
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ELISA
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab97554 has been referenced in 5 publications.
- Rothbarth J et al. Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo. Biochem Pharmacol 67:1771-8 (2004). PubMed: 15081876
- Frank AJ & Tilby MJ Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation. Exp Cell Res 283:127-34 (2003). PubMed: 12581733
- Buschfort-Papewalis C et al. Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response. Blood 100:845-53 (2002). PubMed: 12130494
- McCartney H et al. Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro. Chem Res Toxicol 14:71-81 (2001). PubMed: 11170510
- Tilby MJ et al. A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy. Carcinogenesis 16:1895-901 (1995). PubMed: 7634420