Overview

  • Product name
    Membrane Integrity WB Antibody Cocktail
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:


    Outer Membrane - Porin ab14734 (MSA03)
    Intermembrane Space - Cytochrome c ab110325 (MSA06)
    Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)
    Matrix Space - Cyclophilin D ab110324 (MSA04)


    This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D. 


    Cocktail Antibodies:



    • Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
      Amount: 50µg
      Working concentration: 2µg/ml

    • Mouse monoclonal [E11AE12BD4] to Cyclophilin 40 (ab110324):
      Amount: 50µg
      Working concentration: 2µg/ml

    • Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (ab110252):
      Amount: 50µg
      Working concentration: 2µg/ml

    • Mouse monoclonal [37BA11] to Cytochrome C (ab110325):
      Amount: 5µg
      Working concentration: 0.1µg/ml

    • Mouse monoclonal [7H10BD4F9] to ATP5A (ab110273):
      Amount: 100µg
      Working concentration: 4µg/ml

  • Tested applications
    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab110414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting.

Images

  • All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)

    Lane 1 : Rat liver mitos prep #1
    Lane 2 : Rat liver mitos prep #2

    Lysates/proteins at 10 µg per lane.
  • Sample: Mouse whole cell lysate (3T3 SA cell line)
    Loading amount: 20µg
    Gel Running conditions: reduced denaturating (4-12% gradient gel)
    Blocking step: 30 min in 5% milk at room temperature
    Dilution: 1/1000
    Incubation time: Overnight at 4°C or 1 hour at room temperature
    Secondary antibody: Goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
    Detection: ECL

References

This product has been referenced in:
  • Sakamaki JI  et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell 66:517-532.e9 (2017). Read more (PubMed: 28525743) »
  • Horibata Y  et al. Identification of the N-terminal transmembrane domain of StarD7 and its importance for mitochondrial outer membrane localization and phosphatidylcholine transfer. Sci Rep 7:8793 (2017). Read more (PubMed: 28821867) »
See all 8 Publications for this product

Customer reviews and Q&As

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Abreviews
Application: Western Blot
Sample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20ug
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Dilution: 1/1000
Incubation time: over night at 4°C or 1 hour at room temperature
Secondary antibody: non Abcam goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
Detection: ECL

Abcam user community

Verified customer

Submitted Aug 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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