Key features and details
- Rabbit polyclonal to Menin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Menin antibody
See all Menin primary antibodies
DescriptionRabbit polyclonal to Menin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
Recombinant fragment (His-T7-tag) corresponding to Human Menin aa 219-395. (Expressed in E.coli).
GVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKL LWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYY RDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKE FFEVANDVIPNLLKEAASLLEAGEERP
Database link: O00255
- IHC-P: Human cerebrum, stomach and pancreas tissues. WB: Pig liver and kidney lysates; HeLa cell lysate; Recombinant human Menin protein.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232818 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 68 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionEssential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.
Involvement in diseaseDefects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
- Information by UniProt
- MEA 1 antibody
- MEA1 antibody
- MEN 1 antibody
Paraffin-embedded human brain tissue stained for Menin using ab232818 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach tissue stained for Menin using ab232818 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human pancreas tissue stained for Menin using ab232818 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Menin antibody (ab232818) at 2 µg/ml + Recombinant human Menin protein
Predicted band size: 68 kDa
All lanes : Anti-Menin antibody (ab232818) at 2 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig kidney lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 68 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232818 has not yet been referenced specifically in any publications.