Anti-Menin antibody (ab2605)
Key features and details
- Rabbit polyclonal to Menin
- Suitable for: ICC/IF, ChIP, IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Menin antibody
See all Menin primary antibodies -
Description
Rabbit polyclonal to Menin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ChIP, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide (Human) - which represents a portion of the C-terminus of human MEN1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purification notes
Affinity purified using the immunising peptideimmobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| ChIP |
Use at an assay dependent concentration.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/10000 - 1/25000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with Recombinant Menin protein (ab114387).
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
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ICC/IF
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/10000 - 1/25000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).Can be blocked with Recombinant Menin protein (ab114387). |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation. - Information by UniProt
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Database links
- Entrez Gene: 539431 Cow
- Entrez Gene: 483758 Dog
- Entrez Gene: 100722977 Guinea pig
- Entrez Gene: 100050500 Horse
- Entrez Gene: 4221 Human
- Entrez Gene: 17283 Mouse
- Entrez Gene: 100523533 Pig
- Entrez Gene: 29417 Rat
see all -
Alternative names
- MEA 1 antibody
- MEA1 antibody
- MEN 1 antibody
see all
Images
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Western blot - Anti-Menin antibody - ChIP Grade (ab2605)All lanes : Anti-Menin antibody (ab2605) at 0.04 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDaSamples in NETN lysis buffer.
Exposure time: 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)Immunohistochemical analysis of human lung carcinoma tissue labeling Menin with ab2605 at 1 µg/ml. Detection: DAB.
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Immunoprecipitation - Anti-Menin antibody (ab2605)Menin was imunnoprecipitated from 1.0 mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab2605 at 6 µg/ml. Western blot was perfomed from the immunoprecipitate using ab2506 at 0.04 µg/ml.
Lane 1 and 2: 20% of ab2605 IP in HEK-293T whole cell lysate
Lane 3: Control IgG
Exposure time: 1 second
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody - ChIP Grade (ab2605)Immunohistochemical analysis of human breast carcinoma tissue labelling Menin with ab2605 at 1µg/ml. Detection: DAB.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab2605 has been referenced in 20 publications.
- Yoshiji S et al. Alu-Mediated MEN1 Gene Deletion and Loss of Heterozygosity in a Patient with Multiple Endocrine Neoplasia Type 1. J Endocr Soc 4:bvaa051 (2020). PubMed: 32715270
- Lines KE et al. Genetic background influences tumour development in heterozygous Men1 knockout mice. Endocr Connect 9:426-437 (2020). PubMed: 32348957
- Lines KE et al. MiR-15a/miR-16-1 expression inversely correlates with cyclin D1 levels in Men1 pituitary NETs. J Endocrinol N/A:N/A (2018). PubMed: 30389902
- Uraki S et al. Atypical pituitary adenoma with MEN1 somatic mutation associated with abnormalities of DNA mismatch repair genes; MLH1 germline mutation and MSH6 somatic mutation. Endocr J 64:895-906 (2017). PubMed: 28701629
- Shell J et al. SomaticVHLMutation in a Patient With MEN1-Associated Metastatic Pancreatic Neuroendocrine Tumor Responding to Sunitinib Treatment: A Case Report. J Endocr Soc 1:1124-1134 (2017). PubMed: 29264567