Question (16072) | Anti-Menin antibody - ChIP Grade (ab2605)

Go to datasheet (ab2605)

Question

I am enquiring about your menin antiboday (ab2605). I was wondering want the concentration of the primary antibody was used to get such a clear band and what the secondary antibody was and at what concentration was it used. The mentioned reference in the data sheet does not mention the above antibody. Secondly, were the cell lysates used in the figure overexpressing menin or was it endogenous?

Answer

Thank you for your enquiry and my apologies for the delay in replying to you, I have just received further information from the lab to answer your questions. We are not sure of the exact dilution used for the image on the datasheet, it is between 1/1000 - 1/10000 and I would recommend to try a range of dilution when you do the preliminary experiments to determine optimal conditions. The secondary is a standard HRP conjugated anti rabbit antibody. The reference on the datasheet was indeed incorrect, however I found the following references which are specific for this antibody: Milne TA et al. Menin and MLL cooperatively regulate expression of cyclin-dependent kinase inhibitors. Proc Natl Acad Sci U S A 102:749-54 (2005). PubMed: 15640349 Yokoyama A et al. The menin tumor suppressor protein is an essential oncogenic cofactor for MLL-associated leukemogenesis. Cell 123:207-18 (2005). PubMed: 16239140 Hughes CM et al. Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Mol Cell 13:587-97 (2004). PubMed: 14992727 The cells used were not tranfected i.e the samples show endogenous levels of menin. Please do not hesitate to contact me if you require further information,

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