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Phone call requesting information regarding the region recognised by Anti-Menin antibody ab2605.
Asked on Jan 26 2012
Thank you for contacting us.
I have looked into whether the anti-Menin antibody (ab2605)would be suitable for the experiments you are hoping to perform. Unfortunately, the immunogen used to raise this antibody is directed against the C-terminal end of human Menin. Through epitope mapping it has been established that the antibody specifically recognises a region betweenresidue 575 and theC-terminus (residue 615) of Menin. This antibody would therefore not be suitable if you are hoping todetect the regionN-terminal of residue 575.
I have however been able to find two other antibodies which we have been raised against peptidesderived from the N-terminal sequence of Menin which may be suitable for you.Ab32963 and ab75923 are both rabbit polyclonals which have been used in Western blotting.A blocking peptide is also available for ab32963, the catalogue code for this is ab32962.
I hope this information has been of help. If you require any further information please do not hesitate to ask.
Answered on Jan 26 2012