Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Menin antibody [EPR3986] (ab92443)

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Overview

  • Product name

    Anti-Menin antibody [EPR3986]
    See all Menin primary antibodies

  • Description

    Rabbit monoclonal [EPR3986] to Menin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Menin aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T, K-562, A431 and HeLa cell lysates; Human colon tissue; Human papillary carcinoma of thyroid gland; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3986

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.

    • Tissue specificity

      Ubiquitous.

    • Involvement in disease

      Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
      Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
    • Information by UniProt

    • Database links

      • Alternative names

        • MEA 1 antibody
        • MEA1 antibody
        • MEN 1 antibody
        • Men1 antibody
        • MEN1_HUMAN antibody
        • Menin antibody
        • Multiple Endocrine Adenomatosis 1 antibody
        • Multiple Endocrine Neoplasia 1 antibody
        • SCG 2 antibody
        • SCG2 antibody
        • Suppressor Candidate Gene 2 antibody
        • Wermer syndrome antibody
        • ZES antibody
        • Zollinger Ellison Syndrome antibody
        see all

      Images

      • Western blot - Anti-Menin antibody [EPR3986] (ab92443)
        Western blot - Anti-Menin antibody [EPR3986] (ab92443)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: Menin knockout HAP1 cell lysate (20 µg)
        Lane 3: Jurkat cell lysate (20 µg)
        Lane 4: A431 cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab92443 observed at 74 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab92443 was shown to recognize Menin when Menin knockout samples were used, along with additional cross-reactive bands. Wild-type and Menin knockout samples were subjected to SDS-PAGE. ab92443 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

      • Western blot - Anti-Menin antibody [EPR3986] (ab92443)
        Western blot - Anti-Menin antibody [EPR3986] (ab92443)
        All lanes : Anti-Menin antibody [EPR3986] (ab92443) at 1/10000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : K-562 cell lysate
        Lane 4 : A431 cell lysate
        Lane 5 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 68 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

        ab92443, at 1/100 dilution, staining Menin in paraffin-embedded (A) Human colon tissue and (B) Human papillary carcinoma of thyroid gland by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443)
        Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443)
        ab92443, at 1/250 dilution, staining Menin in HeLa cells by Immunofluorescence.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

        ab92443 showing positive staining in Breast carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

        ab92443 showing positive staining in Colonic adenocarcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

        ab92443 showing positive staining in Lung adenocarcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

        ab92443 showing positive staining in Glioma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Wang J  et al. Menin mediates Tat-induced neuronal apoptosis in brain frontal cortex of SIV-infected macaques and in Tat-treated cells. Oncotarget 8:18082-18094 (2017). WB ; Rhesus monkey . Read more (PubMed: 28178646) »
      • Hamze Z  et al. Altered MENIN expression disrupts the MAFA differentiation pathway in insulinoma. Endocr Relat Cancer 20:833-48 (2013). IHC ; Human . Read more (PubMed: 24157940) »
      See all 2 Publications for this product

      Publishing research using ab92443? Please let us know so that we can cite the reference in this datasheet.

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