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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Menin antibody [EPR3986] (ab92443)

Submit a review Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
  • Western blot - Anti-Menin antibody [EPR3986] (ab92443)
  • Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443)
  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)
  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)
  • Western blot - Anti-Menin antibody [EPR3986] (ab92443)
  • Anti-Menin antibody [EPR3986] (ab92443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3986] to Menin
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Menin antibody [EPR3986]
    See all Menin primary antibodies

  • Description

    Rabbit monoclonal [EPR3986] to Menin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Menin aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Wild-type HAP1, Jurkat cell lysates. IP: Jurkat whole and MEF cell lysates. ICC/IF: Jurkat cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3986

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use between 1/10,000-1/50,000.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use between 1/100-1/250.
ICC/IF
1/50.

For unpurified use between 1/250-1/500.
Notes
WB
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use between 1/10,000-1/50,000.
IP
1/10 - 1/100.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use between 1/100-1/250.
ICC/IF
1/50.

For unpurified use between 1/250-1/500.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
    Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MEA 1 antibody
    • MEA1 antibody
    • MEN 1 antibody
    • Men1 antibody
    • MEN1_HUMAN antibody
    • Menin antibody
    • Multiple Endocrine Adenomatosis 1 antibody
    • Multiple Endocrine Neoplasia 1 antibody
    • SCG 2 antibody
    • SCG2 antibody
    • Suppressor Candidate Gene 2 antibody
    • Wermer syndrome antibody
    • ZES antibody
    • Zollinger Ellison Syndrome antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labelling Menin with purified ab92443 at 1/100 dilution (6.08 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling Menin with purified ab92443 at 1/100 dilution (6.08 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labelling Menin with purified ab92443 at 1/500 dilution (1.22 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Menin antibody [EPR3986] (ab92443)
    Western blot - Anti-Menin antibody [EPR3986] (ab92443)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Menin knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92443 observed at 74 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab92443 was shown to recognize Menin when Menin knockout samples were used, along with additional cross-reactive bands. Wild-type and Menin knockout samples were subjected to SDS-PAGE. ab92443 and ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443)
    Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443)

    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Menin with purified ab92443 at 1/50 dilution (12.1 µg/mL). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.6 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150078) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)
    Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)

    Purified ab92443 at 1/50 dilution (2µg) immunoprecipitating Menin in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab92443 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92443 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 75 kDa

  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)
    Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)

    Menin was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 ug with ab92443 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab92443 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 μg

    Lane 2: ab92443 IP in MEF whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab92443 in MEF whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

  • Western blot - Anti-Menin antibody [EPR3986] (ab92443)
    Western blot - Anti-Menin antibody [EPR3986] (ab92443)
    All lanes : Anti-Menin antibody [EPR3986] (ab92443) at 1/1000 dilution (Purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Anti-Menin antibody [EPR3986] (ab92443)
    Anti-Menin antibody [EPR3986] (ab92443)

References (5)

Publishing research using ab92443? Please let us know so that we can cite the reference in this datasheet.

ab92443 has been referenced in 5 publications.

  • Bullerwell CE  et al. EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex. Sci Rep 11:1537 (2021). PubMed: 33452395
  • Hu WM  et al. Identification of Novel Variants in MEN1: A Study Conducted with Four Multiple Endocrine Neoplasia Type 1 Patients. Horm Metab Res 52:788-795 (2020). PubMed: 32299109
  • Zhu H  et al. The clinical characteristics and molecular mechanism of pituitary adenoma associated with meningioma. J Transl Med 17:354 (2019). PubMed: 31665029
  • Wang J  et al. Menin mediates Tat-induced neuronal apoptosis in brain frontal cortex of SIV-infected macaques and in Tat-treated cells. Oncotarget 8:18082-18094 (2017). WB ; Rhesus monkey . PubMed: 28178646
  • Hamze Z  et al. Altered MENIN expression disrupts the MAFA differentiation pathway in insulinoma. Endocr Relat Cancer 20:833-48 (2013). IHC ; Human . PubMed: 24157940

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