Anti-MEOX 2 antibody (ab174116)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-MEOX 2 antibody
See all MEOX 2 primary antibodies -
DescriptionRabbit polyclonal to MEOX 2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human MEOX 2 aa 163-192 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P50222 -
Positive control
- NIH-3T3 cells; MDA-MB453 cell line lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesab174116 is purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab174116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | 1/10 - 1/50. | |
WB | 1/100 - 1/500. Predicted molecular weight: 34 kDa. |
Target
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FunctionRole in mesoderm induction and its earliest regional specification, somitogenesis, and myogenic and sclerotomal differentiation. May have a regulatory role when quiescent vascular smooth muscle cells reenter the cell cycle.
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Tissue specificityEmbryo and placenta.
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Sequence similaritiesContains 1 homeobox DNA-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 4223 Human
- Entrez Gene: 17286 Mouse
- Entrez Gene: 29279 Rat
- Omim: 600535 Human
- SwissProt: P50222 Human
- SwissProt: P32443 Mouse
- SwissProt: P39020 Rat
- Unigene: 170355 Human
see all -
Alternative names
- GAX antibody
- Growth arrest-specific homeobox antibody
- Homeobox protein MOX 2 antibody
see all
Images
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Anti-MEOX 2 antibody (ab174116) at 1/100 dilution + MDA-MB453 cell line lysate at 35 µg
Predicted band size: 34 kDa -
Confocal immunofluorescent analysis of NIH-3T3 cells labeling MEOX 2 with ab174116 at 1/25 dilution. NIH-3T3 cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min) prior to incubation with primary antibody (1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used at 1/400, dilution for 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
Datasheets and documents
References
ab174116 has not yet been referenced specifically in any publications.