Anti-MEOX 2 antibody (ab174116)

Confocal immunofluorescent analysis of NIH-3T3 cells labeling MEOX 2 with ab174116 at 1/25 dilution. NIH-3T3 cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min) prior to incubation with primary antibody (1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used at 1/400, dilution for 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).