Anti-MEOX 2 antibody (ab174116)
Key features and details
- Rabbit polyclonal to MEOX 2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEOX 2 antibody
See all MEOX 2 primary antibodies -
Description
Rabbit polyclonal to MEOX 2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human MEOX 2 aa 163-192 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P50222 -
Positive control
- NIH-3T3 cells; MDA-MB453 cell line lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab174116 is purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab174116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/10 - 1/50. | |
| WB | 1/100 - 1/500. Predicted molecular weight: 34 kDa. |
Target
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Function
Role in mesoderm induction and its earliest regional specification, somitogenesis, and myogenic and sclerotomal differentiation. May have a regulatory role when quiescent vascular smooth muscle cells reenter the cell cycle. -
Tissue specificity
Embryo and placenta. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4223 Human
- Entrez Gene: 17286 Mouse
- Entrez Gene: 29279 Rat
- Omim: 600535 Human
- SwissProt: P50222 Human
- SwissProt: P32443 Mouse
- SwissProt: P39020 Rat
- Unigene: 170355 Human
see all -
Alternative names
- GAX antibody
- Growth arrest-specific homeobox antibody
- Homeobox protein MOX 2 antibody
see all
Images
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Western blot - Anti-MEOX 2 antibody (ab174116)Anti-MEOX 2 antibody (ab174116) at 1/100 dilution + MDA-MB453 cell line lysate at 35 µg
Predicted band size: 34 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MEOX 2 antibody (ab174116)Confocal immunofluorescent analysis of NIH-3T3 cells labeling MEOX 2 with ab174116 at 1/25 dilution. NIH-3T3 cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min) prior to incubation with primary antibody (1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used at 1/400, dilution for 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
Protocols
Datasheets and documents
References (0)
ab174116 has not yet been referenced specifically in any publications.
