Key features and details
- Rabbit polyclonal to MEOX 2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MEOX 2 antibody
See all MEOX 2 primary antibodies
DescriptionRabbit polyclonal to MEOX 2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human MEOX 2 aa 163-192 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P50222
- NIH-3T3 cells; MDA-MB453 cell line lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab174116 is purified through a protein A column, followed by peptide affinity purification.
Our Abpromise guarantee covers the use of ab174116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|WB||1/100 - 1/500. Predicted molecular weight: 34 kDa.|
FunctionRole in mesoderm induction and its earliest regional specification, somitogenesis, and myogenic and sclerotomal differentiation. May have a regulatory role when quiescent vascular smooth muscle cells reenter the cell cycle.
Tissue specificityEmbryo and placenta.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
- Information by UniProt
- GAX antibody
- Growth arrest-specific homeobox antibody
- Homeobox protein MOX 2 antibody
Anti-MEOX 2 antibody (ab174116) at 1/100 dilution + MDA-MB453 cell line lysate at 35 µg
Predicted band size: 34 kDa
Confocal immunofluorescent analysis of NIH-3T3 cells labeling MEOX 2 with ab174116 at 1/25 dilution. NIH-3T3 cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min) prior to incubation with primary antibody (1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used at 1/400, dilution for 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
ab174116 has not yet been referenced specifically in any publications.