Key features and details
- Rabbit polyclonal to MEPCE - C-terminal
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MEPCE antibody - C-terminal
See all MEPCE primary antibodies
DescriptionRabbit polyclonal to MEPCE - C-terminal
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
- 293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab185991 was affinity purified using an epitope specific to MEPCE immobilized on solid support.
Our Abpromise guarantee covers the use of ab185991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceMEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.
- 7SK snRNA methylphosphate capping enzyme antibody
- BCDIN3 antibody
- Bicoid interacting protein 3 homolog antibody
Detection of MEPCE in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185991 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-MEPCE antibody was used at 1µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab185991 has been referenced in 1 publication.
- Egloff S et al. The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression. EMBO J 36:934-948 (2017). PubMed: 28254838