Overview

  • Product name

    Anti-MEPCE antibody - C-terminal
    See all MEPCE primary antibodies
  • Description

    Rabbit polyclonal to MEPCE - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human MEPCE aa 639-689 (C terminal). The exact sequence is proprietary. NP_062552.2.
    Sequence:

    RI QLKPEQFSSY LTSPDVGFSS YELVATPHNT SKGFQRPVYL FHKARSPSH


    Database link: Q7L2J0

  • Positive control

    • 293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab185991 was affinity purified using an epitope specific to MEPCE immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.
    • Database links

    • Alternative names

      • 7SK snRNA methylphosphate capping enzyme antibody
      • BCDIN3 antibody
      • Bicoid interacting protein 3 homolog antibody
      • Bin3 bicoid interacting 3 antibody
      • Bin3 bicoid interacting 3 homolog (Drosophila) antibody
      • Bin3 bicoid interacting 3 homolog antibody
      • Bin3 homolog antibody
      • FLJ20257 antibody
      • MePCE antibody
      • Methylphosphate capping enzyme antibody
      see all

    Images

    • Detection of MEPCE in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185991 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-MEPCE antibody was used at 1µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    This product has been referenced in:

    • Egloff S  et al. The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression. EMBO J 36:934-948 (2017). Read more (PubMed: 28254838) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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