Key features and details
- Rabbit polyclonal to Meprin beta
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Meprin beta antibody
See all Meprin beta primary antibodies
DescriptionRabbit polyclonal to Meprin beta
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- RT-4, U-251 MG, Human plasma, Human liver and Human tonsil lysates; Human small intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 80 kDa.|
FunctionMembrane metallopeptidase that sheds many membrane-bound proteins. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.
Tissue specificityThe major site of expression is the brush border membrane of small intestinal and kidney epithelial cells.
Sequence similaritiesBelongs to the peptidase M12A family.
Contains 1 EGF-like domain.
Contains 1 MAM domain.
Contains 1 MATH domain.
modificationsN-glycosylated; contains high mannose and/or complex biantennary structures.
O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human.
Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues.
Cellular localizationCell membrane. Secreted. Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17.
- Information by UniProt
- Endopeptidase 2 antibody
- Endopeptidase-2 antibody
- kinase splitting membranal proteinase antibody
All lanes : Anti-Meprin beta antibody (ab204886) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 80 kDa
Immunohistochemical analysis of Paraffin-embedded Human small intestine labeling Meprin beta with ab204886 at 1/20 dilution.
ab204886 has not yet been referenced specifically in any publications.