Key features and details
- Rabbit polyclonal to Meprin beta
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Meprin beta antibody
See all Meprin beta primary antibodies
DescriptionRabbit polyclonal to Meprin beta
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Recombinant fragment (His-tag) corresponding to Rat Meprin beta aa 433-679. N-terminal tag. Expressed in E. coli.
WHIQNFTQLLGGQTTVYSPPFYSSKGYAFQINLDLTSPTNVGLYFHLISG ANDDQLQWPCPWQQATMTLLDQNPDIRQRMSNQRSITTDPKMTDDNGSYL WDRPSKVGVEAFFPNGTQFSRGRGYGTSVFITQERLKSREFLKGDDVYIL LTVEDISHLNSTAAVPGPVPTTSTVHNACSEVECQNGGICTLQEGRAECK CPAGEDWWYMGKRCEKRGSTKDTIVIAVSSTVTVFAVMLIITLISVY
Database link: P28826
- WB: Recombinant rat Meprin beta protein; rat kidney and intestine tissue lysate. IHC-P: Rat liver and kidney tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.05% Proclin 300, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
Our Abpromise guarantee covers the use of ab231543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 79 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionMembrane metallopeptidase that sheds many membrane-bound proteins. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.
Tissue specificityThe major site of expression is the brush border membrane of small intestinal and kidney epithelial cells.
Sequence similaritiesBelongs to the peptidase M12A family.
Contains 1 EGF-like domain.
Contains 1 MAM domain.
Contains 1 MATH domain.
modificationsN-glycosylated; contains high mannose and/or complex biantennary structures.
O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human.
Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues.
Cellular localizationCell membrane. Secreted. Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17.
- Information by UniProt
- Endopeptidase 2 antibody
- Endopeptidase-2 antibody
- kinase splitting membranal proteinase antibody
Formalin-fixed, paraffin-embedded rat liver tissue stained for Meprin beta using ab231543 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat kidney tissue stained for Meprin beta using ab231543 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-Meprin beta antibody (ab231543) at 5 µg/ml + Recombinant rat Meprin beta protein
Predicted band size: 79 kDa
All lanes : Anti-Meprin beta antibody (ab231543) at 3 µg/ml
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat intestine tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 79 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231543 has not yet been referenced specifically in any publications.