Product nameAnti-MER + SKY antibody
DescriptionRabbit polyclonal to MER + SKY
SpecificityDetects endogenous levels of total MER + SKY protein.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic non-phosphopeptide derived from human MER + SKY around the phosphorylation site of tyrosine 749/681 (K-I-YP-S-G).
- WB: extracts from 3T3 cells treated with EGF (200ng/ml, 5mins); IHC-P: Human brain tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 110 or 97 kDa (MER and SKY respectively).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMER is a member of the MER/AXL/SKY receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). SKY may be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.
Cellular localizationMembrane; Single-pass type I membrane protein
- AI323366 antibody
- Brt antibody
- Bruton agammaglobulinemia tyrosine kinase antibody
Left panel: immunohistochemistry analysis of paraffin-embedded Human brain tissue using 1/50 ab79223. Right panel: same, after pre-incubation with the immunizing peptide.
All lanes : Anti-MER + SKY antibody (ab79223) at 1/500 dilution
Lane 1 : 3T3 cell extract treated
with EGF (200ng/ml, 5mins)
Lane 2 : 3T3 cell extract treated
with EGF (200ng/ml, 5mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 , 97 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 150 kDa. We are unsure as to the identity of these extra bands.
ab79223 has not yet been referenced specifically in any publications.