Anti-MERTK antibody (ab95925)
Key features and details
- Rabbit polyclonal to MERTK
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-MERTK antibody
See all MERTK primary antibodies -
Description
Rabbit polyclonal to MERTK -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse MERTK aa 900 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab106208) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: NIH 3T3; RAW 264.7. This antibody gave a positive signal in the following Mouse tissue lysates: Brain, Spleen, Kidney.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 µg/ml. Detects a band of approximately 150, 90 kDa (predicted molecular weight: 110 kDa). Abcam recommends using milk as the blocking agent. |
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ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
Involvement in disease
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 17289 Mouse
- Entrez Gene: 65037 Rat
- SwissProt: Q60805 Mouse
- SwissProt: P57097 Rat
- Unigene: 239655 Mouse
- Unigene: 48789 Rat
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Alternative names
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
Images
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ICC/IF image of ab95925 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95925 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-MERTK antibody (ab95925) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse) Whole Cell Lysate
Lane 2 : RAW 264.7 (Mouse) Whole Cell Lysate
Lane 3 : Spleen (Mouse) Tissue Lysate
Lane 4 : Brain (Mouse) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 150,190 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab95925 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Although MERTK has a predicted mw of 110 kDa, it has been shown to migrate at 150-190 kDa due to glycosylation, dependant upon the tissue in which it is expressed (J. Biol. Chem., 277, 17016-17022).
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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All lanes : Anti-MERTK antibody (ab95925) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
MERTK protein contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References (4)
ab95925 has been referenced in 4 publications.
- Sayama A et al. UNC569-induced Morphological Changes in Pigment Epithelia and Photoreceptor Cells in the Retina through MerTK Inhibition in Mice. Toxicol Pathol 46:193-201 (2018). PubMed: 29310530
- Suresh Babu S et al. MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes. Sci Rep 6:36207 (2016). IP . PubMed: 27827458
- Heller JP et al. A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases. Front Cell Neurosci 9:449 (2015). PubMed: 26635529
- Munday DC et al. Proteomic analysis of mitochondria in respiratory epithelial cells infected with human respiratory syncytial virus and functional implications for virus and cell biology. J Pharm Pharmacol 67:300-18 (2015). PubMed: 25533920