Recombinant Anti-MERTK antibody [EPR17534-139] (ab184086)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17534-139] to MERTK
- Suitable for: IHC-Fr, WB, IHC-P, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-MERTK antibody [EPR17534-139]
See all MERTK primary antibodies -
Description
Rabbit monoclonal [EPR17534-139] to MERTK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 cell lysate; Mouse spleen and liver tissue lysate. IHC-P: Mouse liver and spleen tissue. IP: Mouse spleen tissue lysate. IHC-Fr: Mouse frozen spleen and liver tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17534-139 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use a concentration of 0.1 - 0.5 µg/ml.
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WB |
1/1000. Predicted molecular weight: 110 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/40.
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Notes |
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IHC-Fr
Use a concentration of 0.1 - 0.5 µg/ml. |
WB
1/1000. Predicted molecular weight: 110 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/40. |
Target
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Function
In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
Involvement in disease
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 17289 Mouse
- SwissProt: Q60805 Mouse
- Unigene: 239655 Mouse
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Alternative names
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
Images
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IHC image of MERTK staining in a section of frozen mouse normal liver performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab184086, 0.5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
IHC image of MERTK staining in a section of frozen mouse normal spleen performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab184086, 0.1ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-MERTK antibody [EPR17534-139] (ab184086) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110-140,180-210 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile observed is consistent with what has been described in the literature (PMID: 17047157).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MERTK with ab184086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous staining on mouse spleen (PMID: 19631584) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MERTK with ab184086 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on hepatic sinusoids of mouse liver (PMID: 23799121) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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MERTK was immunoprecipitated from 10 μg of mouse spleen tissue lysate with ab184086 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184086 at 1/1000 dilution. Secondary used is VeriBlot for IP Detection Reagent (HRP) (ab131366) at a 1/10,000 dilution.
Lane 1: Mouse spleen tissue lysate, 10 μg (input).
Lane 2: ab184086 IP in mouse spleen tissue lysate (+)
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184086 in mouse spleen lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 5 secondsThe molecular masses observed are consistent with what has been described in the literature. The band at approximately 50 kDa likely represents a cleavage fragment (PMID: 17047157, 15673687).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab184086 has been referenced in 2 publications.
- Wu H et al. Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury. J Neuroinflammation 18:2 (2021). PubMed: 33402181
- Shi W et al. Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis. Biosci Rep 41:N/A (2021). PubMed: 33969376