Product nameAnti-MERTK antibody [EPR23248-259] - BSA and Azide free
See all MERTK primary antibodies
DescriptionRabbit monoclonal [EPR23248-259] to MERTK - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human MERTK aa 1-550. The exact sequence is proprietary.
Database link: Q12866
- WB: HEK-293, HepG2, U-937, Human spleen, Human ovary, Human testis, Human liver, Mouse spleen, Mouse kidney and Rat spleen, Rat kidney lysates.
ab272177 is the carrier-free version of ab270448. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab272177 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab272177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.|
FunctionIn case of filovirus infection, seems to function as a cell entry factor.
Tissue specificityNot expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
Involvement in diseaseDefects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
All lanes : Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution
Lane 1 : Human ovary tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 150, 210 kDa why is the actual band size different from the predicted?
Lane 1: 3 minutes
Lane 2: 70 seconds
Blocking/Diluting buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270448).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272177 has not yet been referenced specifically in any publications.