• Product name
    Anti-MERTK antibody [Y323]
    See all MERTK primary antibodies
  • Description
    Rabbit monoclonal [Y323] to MERTK
  • Host species
  • Tested applications
    Suitable for: Blocking, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MERTK aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: HEK-293 and K562 cell lysate. IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration. PubMed: 19204785
WB 1/2000. Detects a band of approximately 180 kDa.

For unpurified use at 1/500.

IP 1/20.

For unpurified use at 1/50.

IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      In case of filovirus infection, seems to function as a cell entry factor.
    • Tissue specificity
      Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
    • Involvement in disease
      Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • c MER antibody
      • c mer proto oncogene tyrosine kinase antibody
      • c-mer antibody
      • cMER antibody
      • cmer protooncogene tyrosine kinase antibody
      • Eyk antibody
      • MER antibody
      • MER receptor tyrosine kinase antibody
      • MERK antibody
      • MERPEN antibody
      • Mertk antibody
      • MERTK c-mer proto-oncogene tyrosine kinase antibody
      • MERTK_HUMAN antibody
      • MGC133349 antibody
      • nmf12 antibody
      • Nyk antibody
      • Proto oncogene tyrosine protein kinase MER antibody
      • Proto oncogene tyrosine protein kinase MER precursor antibody
      • Proto-oncogene c-Mer antibody
      • Receptor tyrosine kinase MerTK antibody
      • RP38 antibody
      • STK kinase antibody
      • Tyrosine-protein kinase Mer antibody
      see all


    • All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/500 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : MERTK knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Lanes 1 - 2: Merged signal (red and green). Green - ab52968 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab52968 was shown to specifically react with MERTK in wild-type HAP1 cells as signal was lost in MERTK knockout cells. Wild-type and MERTK knockout samples were subjected to SDS-PAGE. The membrane was blocked with 0. Ab52968 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab52968 used at a 1/200 dilution, 18 hours at 4°C, 5% BSA in Tween PBS.

      Positive cell line: A172

      Negative cell lines: CV1 and HeLa.

      Secondary used is a goat anti-rabbit polyclonal-HRP at 1/10000 dilution.

      Blocked with 5% milk for 1 hour at RT.

      See Abreview

    • All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution (purified)

      Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Observed band size: 140-180 kDa
      why is the actual band size different from the predicted?

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

      Lane 2 - PBS.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

      Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

      Negative control using PBS instead of primary antibody (Inset).

    • Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Observed band size: 180 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.


    This product has been referenced in:
    • Frank AC  et al. Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype. Nat Commun 10:1135 (2019). Read more (PubMed: 30850595) »
    • Lee-Sherick AB  et al. MERTK inhibition alters the PD-1 axis and promotes anti-leukemia immunity. JCI Insight 3:N/A (2018). Read more (PubMed: 30385715) »
    See all 31 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Nov 14 2017


    Unfortunately, in few cases we cannot find the connection between the antibody used in a publication and the antibody used in our catalog, especially as we are trying to link this kind of information to the datasheets.
    However, as we have only two matching isotypes in this case, and both differ only in the amount we sell:
    https://www.abcam.com/index.html?datasheet=125938 https://www.abcam.com/index.html?datasheet=125938.
    https://www.abcam.com/index.html?datasheet=128142 https://www.abcam.com/index.html?datasheet=128142.

    Read More


    Thank you for contacting us and your interest in our products.

    I can now confirm that the concentration of the anti-MERTK antibody [Y323] (ab52968), of the lot number GR54920-5 is 0.1180 mg/ml.

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

    Read More


    Thank you for contacting us and sorry for the delay of our reply.
    Please find below the reply I just received from the source of the anti-MERTK antibody [Y323], ab52968:
    > "Sorry for the delay in response. As stated previously, the anti-MERTK antibody ab52968 displayed clear membranous and some cytoplasmic IHC staining of human lymphoma tissue.
    > Yes, it is true despite the region of the immunogen.
    > The image is from formalin-fixed paraffin-embedded tissue, and we performed in-house testing.
    > Although we do not have a paper for IHC, the antibody's specificity is demonstrated in WB and IF.
    > Unfortunately, that is all the information we could find."
    I appreciate the time you have spent on these experiments and would be pleased to arrange, if you agree, a replacement in compensation. The lot we currently have in stock is GR54920. Let me know if you received a different lot so I could send this one to you. Also, could you please inform me when the antibody was ordered and if it was ordered via a distributor?
    Alternatively I can send you another anti-MERTK antibody that could maybe confirm the results you obtained with ab52968. The link to the list of the 9 anti-MERTK we have is: https://www.abcam.com/index.html?t=17227&pt=1
    We would like to investigate this issue much further, so could you please provide some information about your protocol by filling in the attached questionnaire?
    I look forward to your reply and I hope this case will be resolved as quickly as possible.
    Thank you for your cooperation.

    Read More


    I just wanted to let you know that we are still waiting for a reply from the originator of this product. I have sent a reminder and I hope we will receive the details about the IHC image you requested in the very near future.
    I am very sorry for the delay. I will forward to you the information as soon as I receive it. Thank you for your patience.

    Read More


    Thank you for contacting us. I apologise for the waiting.
    Anti-MERTK antibody [Y323] (ab52968) displayed clear membranous and some cytoplasmic IHC staining of human lymphoma tissue (see larger image attached).
    The Human Protein Atlas states “Most normal tissues showed moderate to strong cytoplasmic positivity with a granular pattern” (see image from the link below).
    I also found another example of MER cytoplasmic staining in IHC (attached).
    I hope this information was helpful. Please do not hesitate to contact us back for further assistance.

    Read More
    Western blot
    Human Cell lysate - whole cell (cell lines)
    Loading amount
    20 µg
    cell lines
    Gel Running Conditions
    Reduced Denaturing (4-15% Tris-glycine gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Mr. Nir Drayman

    Verified customer

    Submitted Mar 05 2012


    Thank you for contacting Abcam. The antibody, ab52968, anti-MERTK antibody [Y323], is covered under our Abpromise, which means that if the antibody does not work as stated on the data sheet and if our support specialist cannot help resolve the issue, then we will provide you with a free of charge replacement or a refund. In this case it is guranteed to work in flow cytometry in mouse tissue. I hope this helps assure about our products and our guaranteed . If there is anything else I can do, please let me know.

    Read More

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