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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MERTK antibody [Y323] (ab52968)

  • Datasheet
  • SDS
Reviews (3)Q&A (6)References (44)

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Western blot - Anti-MERTK antibody [Y323] (ab52968)
  • Western blot - Anti-MERTK antibody [Y323] (ab52968)
  • Western blot - Anti-MERTK antibody [Y323] (ab52968)
  • Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)
  • Immunohistochemistry (Frozen sections) - Anti-MERTK antibody [Y323] (ab52968)
  • Western blot - Anti-MERTK antibody [Y323] (ab52968)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)
  • Anti-MERTK antibody [Y323] (ab52968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y323] to MERTK
  • Suitable for: WB, IP, IHC-P, IHC-Fr
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-MERTK antibody [Y323]
    See all MERTK primary antibodies
  • Description

    Rabbit monoclonal [Y323] to MERTK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MERTK aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293 and K562 cell lysate. IHC-P: Human tonsil and lymphoma tissues. IP: MERTK IP in HEK-293 cell lysate. IHC-Fr: Hu Spleen tissue sections.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y323
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Sensory System
    • Visual system

Associated products

  • Alternative Versions

    • Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
    • HRP Anti-MERTK antibody [Y323] (ab197643)
    • Anti-MERTK antibody [Y323] - BSA and Azide free (ab271851)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HEK-293 whole cell lysate (ab7902)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human MERTK (mutated A708S) protein (ab167957)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/2000. Detects a band of approximately 180 kDa.

For unpurified use at 1/500.

IP
1/20.

For unpurified use at 1/50.

IHC-P (2)
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IHC-Fr
Use at an assay dependent concentration.
Notes
WB
1/2000. Detects a band of approximately 180 kDa.

For unpurified use at 1/500.

IP
1/20.

For unpurified use at 1/50.

IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

IHC-Fr
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      In case of filovirus infection, seems to function as a cell entry factor.
    • Tissue specificity

      Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
    • Involvement in disease

      Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q12866 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10461 Human
      • Omim: 604705 Human
      • SwissProt: Q12866 Human
      • Unigene: 306178 Human
      • Alternative names

        • c MER antibody
        • c mer proto oncogene tyrosine kinase antibody
        • c-mer antibody
        • cMER antibody
        • cmer protooncogene tyrosine kinase antibody
        • Eyk antibody
        • MER antibody
        • MER receptor tyrosine kinase antibody
        • MERK antibody
        • MERPEN antibody
        • Mertk antibody
        • MERTK c-mer proto-oncogene tyrosine kinase antibody
        • MERTK_HUMAN antibody
        • MGC133349 antibody
        • nmf12 antibody
        • Nyk antibody
        • Proto oncogene tyrosine protein kinase MER antibody
        • Proto oncogene tyrosine protein kinase MER precursor antibody
        • Proto-oncogene c-Mer antibody
        • Receptor tyrosine kinase MerTK antibody
        • RP38 antibody
        • STK kinase antibody
        • Tyrosine-protein kinase Mer antibody
        see all

      Images

      • Western blot - Anti-MERTK antibody [Y323] (ab52968)
        Western blot - Anti-MERTK antibody [Y323] (ab52968)
        All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/500 dilution

        Lane 1 : Wild-type HAP1 whole cell lysate
        Lane 2 : MERTK knockout HAP1 whole cell lysate

        Lysates/proteins at 20 µg per lane.


        Lanes 1 - 2: Merged signal (red and green). Green - ab52968 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab52968 was shown to specifically react with MERTK in wild-type HAP1 cells as signal was lost in MERTK knockout cells. Wild-type and MERTK knockout samples were subjected to SDS-PAGE. The membrane was blocked with 0. Ab52968 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-MERTK antibody [Y323] (ab52968)
        Western blot - Anti-MERTK antibody [Y323] (ab52968)

        ab52968 used at a 1/200 dilution, 18 hours at 4°C, 5% BSA in Tween PBS.

        Positive cell line: A172

        Negative cell lines: CV1 and HeLa.

        Secondary used is a goat anti-rabbit polyclonal-HRP at 1/10000 dilution.

        Blocked with 5% milk for 1 hour at RT.

        See Abreview

      • Western blot - Anti-MERTK antibody [Y323] (ab52968)
        Western blot - Anti-MERTK antibody [Y323] (ab52968)
        All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution (purified)

        Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
        Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

        Observed band size: 140-180 kDa why is the actual band size different from the predicted?



        Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

      • Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968)
        Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968)

        Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

        Lane 2 - PBS.

        For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
        Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

        Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

        Negative control using PBS instead of primary antibody (Inset).

      • Immunohistochemistry (Frozen sections) - Anti-MERTK antibody [Y323] (ab52968)
        Immunohistochemistry (Frozen sections) - Anti-MERTK antibody [Y323] (ab52968)

        IHC image of MERTK staining in a section of frozen normal human spleen performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab52968, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      • Western blot - Anti-MERTK antibody [Y323] (ab52968)
        Western blot - Anti-MERTK antibody [Y323] (ab52968)
        Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

        Secondary
        HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Observed band size: 180 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Anti-MERTK antibody [Y323] (ab52968)
        Anti-MERTK antibody [Y323] (ab52968)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (44)

      Publishing research using ab52968? Please let us know so that we can cite the reference in this datasheet.

      ab52968 has been referenced in 44 publications.

      • Vullings J  et al. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis. J Immunol Res 2020:9690832 (2020). PubMed: 32964059
      • Pontara A  et al. Immunohistochemical Evaluation and Clinicopathological Correlation of Mer and Axl Tyrosine Kinase TAM Receptors in Cutaneous Melanoma. Dermatol Pract Concept 10:e2020029 (2020). PubMed: 32363092
      • Zhang Y  et al. Angiotensin II deteriorates advanced atherosclerosis by promoting MerTK cleavage and impairing efferocytosis through the AT1R/ROS/p38 MAPK/ADAM17 pathway. Am J Physiol Cell Physiol 317:C776-C787 (2019). PubMed: 31390228
      • Sinik L  et al. Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma. Mol Cancer Ther 18:278-288 (2019). PubMed: 30482852
      • Simonin Y  et al. Zika virus induces strong inflammatory responses and impairs homeostasis and function of the human retinal pigment epithelium. EBioMedicine 39:315-331 (2019). PubMed: 30579862
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

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      1-3 of 3 Abreviews

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MERTK antibody [Y323]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Blood vessel-Brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH9
      Permeabilization
      No
      Specification
      Blood vessel-Brain
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 30 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MERTK antibody [Y323]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
      Permeabilization
      No
      Specification
      Lung
      Blocking step
      PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 14 2017

      Western blot abreview for Anti-MERTK antibody [Y323]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (cell lines)
      Loading amount
      20 µg
      Specification
      cell lines
      Gel Running Conditions
      Reduced Denaturing (4-15% Tris-glycine gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Mr. Nir Drayman

      Verified customer

      Submitted Mar 05 2012

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